TaWRKY70转录因子参与小麦抗条锈病机理研究

The main management strategy of controlling wheat stripe rust (Puccinia striiformis f. sp. tritici, Pst) is planting resistant cultivar. Due to the virulence variation of Pst, the cultivar resistance loses quickly. Using the transcription factor to regulate the expression of resistant genes could improve the durability of cultivar resistance. WRKY transcription factor family is one of the important transcription factor families, in whcih WRKY70 is a vital regulator in plant resistance response to pathogen attack. But in wheat, the resistance-related signaling pathways that WRKY70 transcription factor regulate is rarely studied. Previously, we found that TaWRKY70 gene was positively involved in wheat resistance response to Pst infection. On this basis, we will create wheat TaWRKY70 transgenic plant, and screen the plants with higher expression level of TaWRKY70; use RNA-Seq, CHIP-Seq and electrophoresis mobility shift assay (EMSA) techniques to identify the resistance-related genes; verify the functions of key resistance genes. This research will conform the TaWRKY70 transcription factor regulated the mechanism of signaling activation and transduction, illuminate the resistance-related singing pathways that TaWRKY70 involved in, providing theoretical basis for effective utilization of wheat resistant cultivar and carrying out improvement of wheat genetic breeding.

小麦条锈病的主要防治措施为种植抗病品种，但是条锈菌毒性变异使得抗病品种缺乏持久抗病性。利用转录因子调控抗病相关基因的表达，可以提高品种持久抗病性。WRKY转录因子家族是植物天然免疫系统中重要转录因子家族之一，其中WRKY70转录因子在植物抗病反应中起着关键调节子的作用，但是在小麦中，关于WRKY70转录因子对抗病信号的分子调控机理研究较少。项目前期研究发现TaWRKY70转录因子正向参与小麦抗条锈病过程。本研究拟在此基础上，创制TaWRKY70转基因小麦，筛选高表达量植株并对其抗病水平进行评价；利用转录组测序、ChIP-Seq、凝胶电泳迁移等技术手段，挖掘关键抗病基因并研究其功能，从而明确TaWRKY70转录因子对抗病相关信号途径激活与传递的调控机理。在分子水平层面揭示TaWRKY70转录因子在小麦抗条锈病过程中的调控机理，为有效利用小麦品种抗病性，推动小麦抗病性状的遗传改良研究奠定基础。

小麦条锈病是小麦生产中重要的真菌病害，从植物自身免疫角度出发，激活植物自身的免疫系统，是防治病害的长久的可持续的措施。本项目创制了TaWRKY70过表达转基因小麦，以此为材料，利用比较转录组学、Chip、EMSA等技术，鉴定了受TaWRKY70调控的重要靶标基因，解析了TaWRKY70调控的抗病途径。此项研究将为从植物自生免疫系统寻找抗病途径奠定了分子基础，为小麦抗条锈病分子育种奠定了理论基础。