负载BMP-2高活性聚酯基骨修复支架的制备与研究

Developing high osteogenic activity scaffolds for bone repair is the most important way to tackle with the difficulties encountered in the bone repair clinical treatments. To design porous structure scaffolds that are loaded with growth factors from bionic prospective is one of the important ways to fabricate high active bone repair materials. To apply this scheme, this project is based on acquired advantages of activating bone repair materials from our research group and combines advanced 3D printing technology, using poly(glycerol sebacate) (PGS) as base materials which has good bioactivity and favorable vascularization, incorporating BMP-2 loaded environmental responsive mesoporous silica nanoparticles (R-MSNs) to print 3D scaffolds with porous structures, aimed at improving the mechanical properties of scaffolds and active loading and sustainably controlled release of BMP-2. On that basis, effects of different base materials structures, printing parameters, BMP-2 distribution and bioactivity on the mechanical properties and osteogenic activities of 3D scaffolds will be investigated from various angles. This project will construct material properties - growth factors - osteogenic activity relations and theoretical models and it will also lay the foundation for the research and development of tissue regenerating materials.

构建高成骨活性的骨修复支架材料，是解决目前临床骨组织修复材料生物活性低的根本途径。从生物仿生角度设计制备具有多孔结构的支架，进一步负载生长因子是构建高活性骨修复材料的重要手段。为此，本项目充分利用研究团队在骨修复材料活性化修饰方面的优势，结合当前先进的3D生物打印技术，以具有优良生物活性和成血管性能的聚癸二酸甘油酯（PGS）为支架基体，以环境响应型大介孔硅基纳米粒子作为聚合物基体的增强剂以及生长因子BMP-2的固载和长效释放载体，通过打印形成具有三维结构的高活性骨修复支架。在此基础上，从不同的角度深入探讨基体材料和介孔材料的性能及组成、打印过程主要参数、BMP-2的分布和生物活性等对骨修复支架力学性能和成骨活性的影响规律。建立材料特征—生长因子—成骨活性之间的关系和理论模型，并为其它组织引导再生材料的研究开发提供借鉴。

本项目首先制备了具有较高的力学强度和生物相容性的聚癸二酸甘油酯（PGS）材料，进一步以PGS/β-磷酸三钙（β-TCP）为支架基体，结合研究团队在骨修复材料活性化修饰方面的优势，以环境响应型大介孔硅基纳米粒子作为生长因子BMP-2的固载和长效释放载体，通过3D生物打印技术形成具有三维微纳结构的高活性骨修复支架。在此基础上，从不同的角度深入探讨基体材料和介孔材料的性能及结构、打印过程主要参数、BMP-2的分布和生物活性等对骨修复支架成骨活性的影响规律。建立材料特征—生长因子—成骨活性之间的关系和理论模型。项目探索了高活性骨修复支架材料的制备方法，优化构建出可用于BMP-2高活性固载和控释的新型高活性骨修复支架材料。本项目经过3年的执行，共申请发明专利2项，发表相关文章4篇,达到了项目的预定目标。