代谢型谷氨酸受体mGluRs在前庭代偿中的作用及机制

The molecular and biochemical mechanisms of vestibular compensation (VC) is not clear until now. It is hard to provide theoretical evidence for clinical treatment of vertigo. The mGluRs have been shown to play a role with VC. Unilateral labyrinthectomy (UL) has been used as a model for investigating the VC. In our preliminary experiment, we found that mGluRs were expressed in both the Purkinje cells in the flocculus and Purkinje cell terminals in the medial vestibular nucleus(MVN) in the rat. The protein level of the mGluR2, in the ipsilateral flocculus, increased at 1day after UL. Above experimental results suggested mGluRs contribute to the VC, mGluRs may regulate long term depression (LTD) in the flooculus. This project will be used a variety of experimental techniques and methods to explore the roles and mechanisms of mGluRs in rat cerebello-vestibular pathway after UL, in order to further reveal the mechanisms of vestibular compensation, and provide theoretical basis for clinical treatement of patients with vertigo.

前庭代偿的分子生物学机制迄今未明，为临床提供治疗眩晕的理论基础较为困难。动物实验发现前庭中枢mGluRs与前庭代偿有关。单侧迷路破坏术是常用于研究前庭代偿的模型之一。我们新近实验发现在大鼠绒球浦肯野细胞和MVN浦肯野终末有mGluRs的表达。在单侧迷路破坏术后1天，mGluR2在同侧绒球中表达较假手术组升高。上述实验结果提示在小脑前庭通路中mGluRs在前庭代偿的发展中可能具有重要意义和作用。本项目将应用多种实验技术和方法研究大鼠小脑前庭通路中mGluRs在单侧迷路破坏术后的作用及机制，旨在进一步揭示前庭代偿机制，从而为临床治疗眩晕提供理论依据。