适配体修饰的管内固相微萃取-微柱液相色谱/串联质谱检测技术平台的建立及其对藻毒素的选择性富集与检测

Microcystin (MC) is a kind of natural toxins produced by toxic species of cyanobacteria. In recent years, both ecological environment and human health have been seriously affected by cyanobacterial blooms. Microcystins directly affect the safety of drinking water and aquatic products because they are the most universal cyanobacterial toxins and very toxic to human health.. In this project, the new in-tube solid-phase microextraction sorbent based on aptamers for microcystin would be developed and in-tube solid-phase microextraction coupled with microcolumn liquid chromatography / tandem mass spectrometry would be established, a selective enrichment and high sensitivity detection method for microcystins in complex matrices would be completed. In this project, automation and integration about analysis of microcystins would be realized. The study would provide theoretical basis and primary data for the mornitoring and forecasting methodology of microcystins in water resource and aquatic products breeding disasters.

微囊藻毒素（microcystin, 简称 MC）是蓝藻产生的一类天然毒素。近年来，我国蓝藻水华频频爆发，被微囊藻毒素污染的饮用水和水产品给人民群众的健康带来巨大的威胁。在本项目中，利用对藻毒素有特异选择性的适配体修饰管内固相微萃取材料，通过建立管内固相微萃取与微柱液相色谱/串联质谱的在线联用技术平台，完成对复杂基质中藻毒素的选择性富集与高灵敏度检测。通过本项目的实施，不仅建立藻毒素的选择性富集新方法，还将实现藻毒素的样品前处理与分析检测的自动化与一体化，为我国生活和环境用水质量评价、水产品养殖灾情预警提供更多的技术支撑。

因蓝藻水华爆发导致的微囊藻毒素污染问题已经受到了越来越多的关注。在本项目中，针对藻毒素在分析与检测中面临的分离富集方面的热点难点问题，设计和制备了一系列新型藻毒素选择性富集材料并与LC-MS/MS联用，对自来水、太湖水、海水和贝类样品进行检测；制备了一种聚(OMA-co-TMPTMA)整体柱；发明并搭建用于装填适配体修饰的固体吸附材料的前处理装置；利用固相微萃取-色谱/质谱联用平台，对环境水体、食品中的常见污染物多环芳烃和农药进行了样品前处理和高灵敏度检测技术研究。本项目的实施为我国水环境质量评价、水产品质量检测提供更多的技术支撑。项目执行期内共发表SCI 论文 8 篇，其中一区1篇，二区7篇。申请国家发明专利5项，其中已授权2项。基于项目组成员长期的研究成果，成功获得 2016 年福建省科学技术进步二等奖一项《食品快检预处理技术与应用》。