一次性微型固态电化学发光检测器及其在多组份分析中的应用

The aim of this proposal is to fabricate low-cost, simple-structure, practical and high-efficiency miniaturized solid-state electrochemiluminescent (ECL) detectors based on electro-polymerization of pyrrole derivatives of tris(2,2’-bipyridine) ruthenium(II), which could obviate influences of materials, morphology and sizes of electrodes. The obtained detectors could be coupled with microchip electrophoresis or liquid chromatography for ECL determination of separated analytes. On the other hand, we will try the array of such miniaturized ECL detectors for assembly of space-separable ECL visualized detectors based on the advantage of ECL visualization and bipolar electrochemistry. In addition, the detector arrays could be integrated with microfluidic systems or paper-based analytical devices for applications of biochemical assays, such as multi-analyte immunoassay. This study could not only develop novel ECL biosensing and ECL imaging techniques but also facilitate utilization of ECL in the fields of medical diagnosis and biochemical analysis.

本项目拟基于不受电极材料、形貌和尺寸影响的电聚合三联吡啶钌(II)吡咯衍生物，构建价格低廉，结构简单和实用高效的一次性微型化固态电化学发光检测器。其与芯片电泳或液相色谱等分离系统集成，可用于分离产物的电化学发光检测。基于电化学发光其可视化的优势，还可尝试研制无线双极电极阵列式电化学发光检测器，利用电化学发光汇报和分析物传感在空间可分离的优势，同时结合微流控系统或纸基分析系统等，用于开展多组份免疫分析等常规生化分析应用。这一研究不但可以发展新型电化学发光生物传感技术和成像技术，还可促进电化学发光技术在医疗检测和生化分析等领域的应用。

本研究项目主要是研发一种价廉、便捷的双极电极电化学发光检测器，为发展阵列型双极电极以及高通量多组份检测提供技术积累，为制备新型ECL生物识别传感器件，发展多组分检测，ECL可视化分析技术，如ECL成像、ECL比色法等生物传感技术提供技术支持。通过对纳米复合材料的开发，提高检测器发光效率，进而提高检测灵敏度，发展能实际应用的固态ECL器件。促进新型ECL器件在医疗卫生检测、生化分析等领域的应用。主要开展了如下的研究工作：1）构建双极电极电化学发光检测平台；2）多色电化学发光研究；3）电化学发光免疫分析；4）双极电极电化学发光成像免疫检测平台；5）细胞检测与成像分析。 目前，已在知名期刊如ACS Sensors, ACS Omega, Biosensors & Bioelectronics, Chemical Communications., Science Bulletin等上发表SCI论文29篇；申请发明专利11项，授权1项，培养研究生13名。