3D纳米纤维支架作为乳腺癌循环肿瘤细胞捕获/鉴定装置的制备及应用研究

Given the fact that the most important cause of cancer-related death in breast cancer patients is metastasis, detection of circulating tumor cells（CTCs）could provide earlier diagnosis to potentially fatal metastases. In contrast to the other CTC capture approaches, nanostructured materials-based CTC capture/detection approach represents a unique concept that mimics the nature of nanoscale.interactions observed ubiquitously in biology. We introduce a new CTC capture/detection assay based on 3D nanofiber scaffold，which was grafted with cell capture agent (i.e., anti-EpCAM). The following specific aims will be pursued: 1) to fabricate the CTC capture/detection assay，2）to test the capture efficiency of CTC capture/detection assay，and 3）to detect the CTCs captured through the CTC capture/detection assay and to determine the potential application in clinic. The significance of this project is to provide a more efficient method for early diagnosis through fabricating a new CTC capture/detection assay for capturing and detecting CTCs from peripheral blood samples of breast cancer patients.

肿瘤转移为乳腺癌患者死亡的重要原因，而循环肿瘤细胞（CTCs）的检测能够为潜在转移提供早期诊断。与其他CTC富集装置相比较，基于纳米结构材料的CTC捕获/鉴定装置能够呈现一种生物学中纳米尺度相互作用的独特概念。基于我们的前期工作，运用包覆细胞捕获（anti-EpCAM）的3D纳米纤维多孔支架作为新型CTC捕获/鉴定装置。本课题的主要内容是：1）构建CTC捕获/鉴定装置 2）评估CTC捕获/鉴定装置细胞捕获效率 3）鉴定CTC捕获/鉴定装置所捕获的CTCs并研究该装置临床应用性。本课题研究意义为：通过构建用于捕获和鉴定乳腺癌患者周边血液中循环肿瘤细胞的新型CTC捕获/鉴定装置，为乳腺癌患者提供更高效早期诊断的方法。

肿瘤转移为乳腺癌患者死亡的重要原因，我们成功制备的3D纳米纤维多孔支架具有作为新型CTC捕获/检定装置的潜能。此外，纳米微泡可作为一种新型超声增强剂，能够增强肿瘤的造影效果。然而目前已有的造影剂对于肿瘤的检测灵敏度较低。我们制备了均一的纳米尺寸微泡(316.5 ± 9.1 nm，多分散性指数为0.15 ± 0.03, n = 3)，并将AAN结合至纳米微泡能够特异性靶向肿瘤，该造影剂可以呈现很好的造影增强效果，体外实验也验证了良好的增强效果。新型靶向纳米微泡可作为新型靶向造影增强剂，具有癌症早期诊断的潜能。