关节假体微弧氧化掺锌微孔纳米涂层的抗菌性能及其机制研究

Bioactive and antibacterial properties of coatings are extremely important to the success of artificial joint replacement surgery. Therefore, it is significant to seek the ideal coating to reduce the incidence of aseptic loosening and infection after artificial joint replacement surgery. Recently, micro-arc oxidation (MAO), a surface-modified technology, has attracted increasing attention because it shows advantages over traditional technology. The desired microporous nanostructure can be evoked by this technology. Beside, some elements, which possess bioactive and antibacterial properties, can be incorporated directly into microporous nanostructured coating, reducing the incidence of loosening and infection. Since the Zinc ions (Zn) has been confirmed as element possessing not only bioactive but also antibacterial properties, microporous nanostructured Zn-incorporated MAO coating (Zn-MAO) is prepared by MAO technique in the Zn-containing electrolyte, and its bioactive property, as well as the possible signal transduction pathways involved have been detected. In this project, the antibacterial property of the novel coating and its molecular mechanism will been investigated.

人工关节假体涂层的生物活性及抗菌性能对于关节置换手术的成功极为重要。因此，寻求理想的涂层以降低人工关节置换术后无菌性松动松动及感染的发生率具有重要意义。晚近，一种新型的表面改性类的微孔纳米涂层技术-微弧氧化法因存在很多传统技术无法替代的优点而备受重视。采用这一技术既可获得理想的微孔纳米涂层，又可将某些兼具生物活性及抗菌性能的因子直接引入微孔纳米材质中，因此，可降低假体无菌性松动及假体周围感染的风险。鉴于锌离子为一种同时具备促成骨细胞增殖、分化活性及抗菌性能的微量元素，本课题组将其引入微弧氧化微孔纳米涂层中，制备微弧氧化掺锌微孔纳米涂层，并业已证实该涂层的促成骨活性及其信号传导途径。本课题就这一新型涂层的抗菌性能及其发挥抗菌性能的分子机制展开研究。

人工关节假体涂层的生物活性及抗菌性能对于关节置换手术的成功极为重要。因此，寻求理想的涂层以降低人工关节置换术后无菌性松动及感染的发生率具有重要意义。我们的前期研究工作已表明：人工关节微弧氧化掺锌纳米微孔涂层Zn-MAO 涂层具备优良的促成骨活性，本课题主要对层Zn-MAO 涂层的抗菌性能及其可能的机制进行探讨。本研究的结果表明：Zn-MAO 涂层于体内外均能有效地抑制金葡菌的生长，且当锌离子含量为0.574 at.% 时，Zn-MAO 涂层的促成骨活性达到最强。Zn-MAO 涂层通过提高金葡菌葡萄球菌自溶酶 E (Atl E)的表达，同时抑制荚膜脂多糖/黏附素(PS/A)及脂多糖黏附素(PIA)的表达而发挥抗菌作用，且Atl E、PS/A、PIA的表达受脂质S(lipidS)的调控。