基于多酚氧化的界面组装对川陈皮素的长效稳定机制研究

To impact the stability of bioactive substances during processing and storage is one of the important strategies for improving the health properties of foods. The previous stabilization and delivery system of food components showed the bottleneck problems including poor stability, difficult to re-dissolve and low loading capacity, which need design and develop new stabilization systems from different ideas and prospective. Inspired by the phenomenon of interfacial oxidative coupling assembly of polyphenol, the bioactive component first forms aggregates and then absorbs polyphenol on the surface, after which polyphenol coatings are generated based on the surface oxidative polymerization of polyphenol, resulting in high loading capacity and long-term stability of the loaded component. Based on this bio-inspired idea, this research will systematically explore the regulation for the formation of polyphenol coatings under self-oxidation and accelerated oxidation (polyphenol oxidase and environmental factors) conditions. The effect of multi-factors on the stability of polyphenol coatings will be studied by means of ethanol-etched and template method. According to the information, the correlationship between characterization of polyphenol coatings and the long-term stability of nobiletin will be constructed, furthermore, the mechanism for long-term stabilization of nobiletin will be investigated. In this project, the new strategy based on small molecule compounds as coating materials will provide theoretical guidance for further developing this new method and more importantly solving the bottleneck problems in stabilization and delivery systems.

赋予生物活性物质在加工及贮藏过程中的稳定性是增进食品健康属性的重要策略之一。现有食品组分稳态化和递送中存在的稳定性差、难复溶、负载量低的瓶颈问题，需要从新的思路、新的视角出发设计、构建全新的稳态化体系。本项目受植物多酚界面易氧化成膜现象的启示，将待稳定的功能因子首先形成聚集体，而后在其表面吸附上多酚类物质，进而通过表面多酚氧化聚合成膜实现对目标物质的高效负载及长效稳定。基于这一仿生的思路，该项目拟系统探究自氧化及加速氧化条件（多酚氧化酶及环境因素）对多酚膜形成的调控，采用负载物溶出及模板法获得多因素下多酚膜稳定性信息，构建多酚氧化膜性质与模型疏水性功能因子川陈皮素长效稳定性之间的相关性，并探讨其长效稳定川陈皮素的作用机制。本项目提出的以小分子化合物为膜材料构建功能因子稳态化的新策略，可为进一步发展这一新方法、解决功能因子稳定及递送的瓶颈制约提供理论借鉴。

本项目创新性的利用植物多酚原位氧化成膜的性质，在选择的模型功能因子川陈皮素纳米聚集体表面自组装成膜，实现对目标物质的高效负载及长效稳定。系统探究了自氧化、加速氧化（多酚氧化酶、环境因素）及金属离子条件下对多酚膜形成的调控，结合多手段原位监测组装过程，并采用负载物溶出及模板法获得多因素下多酚膜稳定性信息。采用变温红外光谱、X射线光电子能谱等手段系统探究多酚膜长效稳定川陈皮素的作用机制。同时，结合现代生物学手段，评价该运载体系体内体外的生物利用率，并剖析背后机制。课题提出的小分子界面结构化修饰的理念和策略，不仅可为营养稳定和递送研究提供新方向和新思路，也可望对深入理解复杂食品体系下多相多组分相互作用及其调控提供理论参考。