微阵列表面修饰3D纳米支架引导神经干细胞定向迁徙修复脊髓损伤

During the research of surfaces modification to the bio-scaffold, the scientist find by prepare a controllable micropatterned surfaces, which can limit the living microenvironment of the cells, and to guide the growth and migration of the cells. We have successfully construct the bio-scaffolds with controllable micropatterned surfaces to promote the directional growth of neural stem cells, which was of great importance for guide the cells through the damaged areas and for the fuctional regeneration of the injuryed spinal cord. But we found during our research, the two-dimensional surface-cell culture can not imitate the complicated three dimensional microenvironment in vivo, which was also to be the most difficult obstacles for the in vivo application. The newly development accuracy of three dimensional print technique provide useful tools for the interface biology research, which can deepen the two-dimensional surface-cell culture research to a three dimensional studies. So, we prepare for a better design of the micropatterned surfaces in a three dimensional structures, with the methods of three dimensional print technique and newly bio-scaffolds, to prepare the three dimensional bio-scaffolds with controllable micropatterned surfaces. In order to better induction the neural stem cells growth and migrate at specified direction, and to explore this new methods with controllable micropatterned bio-scaffolds for spinal cord repairing.

近年来生物材料表面修饰的研究发现，微阵列技术制备具特定界面构型的ECM支架，对细胞的生长迁徙具特殊引导作用。我们之前的研究已构建具特定可控界面构型的二维生物支架，并成功地引导了神经干细胞的定向生长、迁徙。这对引导细胞穿越脊髓损伤区域修复神经功能具重要意义。 但研究中我们也发现，单纯的二维界面-细胞培养难以模拟体内复杂的三维环境，这也是可控界面构型二维支架难以深入进行体内实验的最大障碍。而最新的高精度3D打印技术的发展为界面生物学的探索性研究提供了有力工具，其可将二维平面的细胞-材料界面研究，深化为可控三维立体空间细胞-界面生物学研究。 因此，我们拟通过对材料界面形态的三维优化设计，利用高精度3D打印技术及具良好生物物理特性的新型生物材料，制备具特定可控界面构型特征的三维ECM支架，以更好的趋向性引导神经干细胞的定向生长、迁徙，探索通过可控界面构型生物支架修复脊髓损伤的新途径。

我们的课题研究，是采用具特定微界面构型的3D纳米支架引导神经干细胞定向迁徙，是通过控制支架材料的空间构型与理化特性来创造特定的细胞生长、迁徙微环境，对于引导细胞定向生长、迁徙，并跨越脊髓损伤区域，从而修复脊髓损伤具有重要的意义。. 在课题的研究中，我们采用多种生物材料，制备供神经干细胞生长、迁徙的支架材料，包括分子自组装多肽、丝素蛋白等材料，构建能影响细胞界面特性，并影响细胞生长迁徙的多种形态的支架界面，并尝试采用高精度生物3D打印技术，可以将细胞预设于特定的材料中，并通过具特定结构的3D打印设计，制备出具特定微界面构型的3D纳米支架，并引导神经干细胞定向迁徙等方便都做了系列研究，后续资料整理后，会发表系列SCI论文。. 在课题研究中，我们按照课题设计，探索了多种细胞-生物支架微观界面的结构形态，并采用不同的细胞进行共培养研究，探索了细胞界面构型对细胞定向迁徙的可能影响，为进一步探讨采用生物3D微结构支架定向引导细胞迁徙，修复脊髓损伤提供理论支撑。. 整个课题研究，严格按计划执行。后续相关资料，会整理发表系列SCI论文，补充上报基金委。