生物矿化荧光磁小体脂质体的微观结构、宏观性质及其功能

A fluorescent magnetic liposome will be synthesized using fluorescent magnetic magnetosomes and lecithin. The fluorescent magnetic liposome is a function drug carry with magnetic controlled drug release and fluorescence tracer, but also is an ideal set of diagnosis-therapy multifunctional system (fluorescence tracer, magnetic targeting, magnetic control drug release, magnetic resonance imaging agent). The fluorescent magnetic magnetosomes will be synthesized by biomineralization-surface modification method. The fluorescent magnetic liposome will be prepared by by supercritical CO2 method. The microstructure information of the fluorescent magnetic liposomes will be obtained by using computer simulation and modern analysis determination method. The macroscopic property will be acquired using microcalorimeter (micro-DSC), vobratomg sample magnetometer (VSM) and various spectral analysis. The function of magnetic control drug release and fluorescence tracer will be studied, and the interaction of the liposome and biosystem will be explored. The results will reveal the relationship between synthesize, preparation, macroscopic property, microstructure and function from the molecular level, and provide the theory basis for the application of biological system.

采用生物矿化-表面改性法制备的荧光磁小体与卵磷脂为原料制备荧光磁性脂质体。该脂质体是一种具有磁控制释药和荧光示踪特殊功能的药物载体，也是一种理想的集诊断、靶向治疗于一体的多功能系统（荧光示踪、磁靶向、磁控制释药、磁共振成像剂）。采用生物矿化-表面改性法制备的荧光磁小体，利用超临界二氧化碳法制备荧光磁性脂质体，使用计算机模拟和现代表征手段获得该脂质体的微观结构，利用微量量热技术(micro-DSC)、振动样品磁强计（VSM）和光谱技术获得它们的宏观性质。研究荧光磁性脂质体的磁控释药、荧光示踪等功能，探讨脂质体与生物体系的相互作用。研究结果将从分子层次揭示荧光磁性脂质体的制备、宏观性质、微观结构和功能。为荧光示踪纳米材料在生物体系中的应用提供理论依据。

趋磁细菌是近年来发现的一种具有磁性的细菌。本项目围绕趋磁细菌的生物合成、改性及其应用展开一系列研究工作。为了完成该研究项目，建立了一系列研究方法。在生物矿化过程中添加荧光碳量子点，获得了荧光趋磁细菌；通过调控生物矿化过程，分别获得具有不同成分和不同的磁学性质的锌-铁磁小体和钴-铁磁小体；成功地采用超临界CO2流体方法分离出具有生物膜包裹的磁小体，并利用磁小体表面的生物膜作为模板用原位碳化法合成了荧光磁小体。研究了锌-磁小体、铁-磁小体和钴-磁小体的形貌和尺寸，表面结构和磁学性质。探讨了荧光磁小体的表面结构、荧光特性和磁学性质,展现了其结构与磁性能的关系；研究了该荧光磁小体的生物相容性和细胞毒性并将该荧光磁小体作为示踪剂负载于脂质体中，探讨和比较了以团簇银脂质体的荧光示踪功能。