葫芦脲薄膜荧光传感器的研究及在药物分析中的应用

Fluorescence analysis with its high sensitivity, good selectivity, simple, and specific advantages has been widely used for the determination of drugs. A variety of film fluorescent sensors for non-fluorescent or weakly fluorescent drugs will be investigated, the development bases on the competitive reaction between drugs and fluorescent probes for occupancy of the cucurbituril cavity. The fluorescent probe molecules which has the inclusion ability with cucurbituril and can be bonded to the film material will be elected. The sensing element will be bonded to the easy-to-film polymer chain by a chemical reaction, a fluorescent sensor with a specific function will be developed. The performance and luminescence behavior of the sensor for molecular recognition of the drugs will be investigated. A new high sensitivity, high selectivity fluorescent sensing method determination of the targeted drug in pharmaceutical preparations and biological fluids will be established. This study gives a new approach to develop fluorescent sensor-based cucurbituril, but also it is useful for promoting fluorescent sensor used in pharmaceutical analysis.

荧光分析法以其灵敏度高、选择性好、操作简便、专属性强的优点目前已被广泛用于药物的测定。本项目旨在以荧光探针与药物在葫芦脲空腔中的竞争反应为基点，研究和开发适合于检测某些无荧光或弱荧光药物的荧光薄膜传感器。筛选出既能与葫芦脲发生包合作用又能键合到薄膜材料上的荧光探针分子，将传感元素通过化学反应键合到高分子薄膜上，研制出具有特定功能的荧光薄膜传感器，研究传感器对药物的识别性能及发光行为。建立某些药物制剂和生物体液中靶向药物的高灵敏度、高选择性的荧光传感新方法。本研究不仅为进一步开发以葫芦脲为受体的荧光传感器开辟一条新的途径，对荧光传感的实际分析应用也起到一定的推动作用。

荧光分析法以其灵敏度高、选择性好、操作简便、专属性强的优点目前已被广泛用于药物的测定。本项目以荧光探针与药物在葫芦脲空腔中的竞争反应为基点，研制出适合于抗癌药物甲氨蝶呤和奥沙利铂的均相荧光传感器，应用于药物制剂和生物体液的荧光测定，通过核磁、理论计算等系统探讨了其分子识别作用机理。其次，采用光聚合和化学共价键合法研制出葫芦脲薄膜传感器，发现此类传感器的识别性能和同类均相传感器的识别有较大的差异，对原来能识别的药物分子不能识别，而对原来不能识别的甲基橙客体分子有较好的识别性能，可研制成动态传感器识别某些药物分子，为此类薄膜传感器器件化的实际应用提供了一定的实验依据。