miR-24通过STING作用于靶向防龋DNA疫苗免疫效应的机制研究

As the newly discovered DNA sensor molecule, STING participates in the immune response of dsDNA viruses or bacteria to regulate the production of type I interferon in the innate immunity as well as to lead to the encoded antigen-specific B cells and T cells . STING was identified as a novel cellular target of miR-24, however, no studies of STING regulated by miR-24 in the immune response of DNA vaccine have been carried out. On the basis of preliminary studies, the role of the miR-24 in the immune response to targeted anti-caries DNA vaccine through STING will be studied in this research. The immune response is explored using the research models of targeted anti-caries DNA vaccine on the immunized mice in vivo and dendritic cell from mice in vitro. The achievement of this study is to further improve the targeted fusion anti-caries DNA vaccine immune response level and to provide new research targets in the future.

作为一种新发现的DNA受体分子，STING参与机体对病毒或细菌的dsDNA 的免疫反应，STING通路通过调节I型干扰素来参与固有免疫，同时引起较强的抗原特异性B细胞，T细胞反应。STING是miR-24可能的靶基因，但尚未有关于miR-24 通过STING信号通路作用于DNA疫苗免疫效应的研究报道。本课题在前期研究的基础上，从以下方面对miR-24通过STING信号通路作用于靶向防龋DNA疫苗免疫效应的机制进行研究：拟以靶向防龋DNA疫苗免疫的小鼠和靶向防龋DNA疫苗刺激的小鼠树突状细胞为研究对象，从体内，体外两方面，对miR-24在疫苗介导的免疫反应中的表达变化，功能意义和作用机制进行研究，为进一步提高靶向防龋DNA疫苗的免疫反应水平提供新的有潜力的研究靶点。

作为一种新发现的DNA受体分子，STING参与机体对病毒或细菌的dsDNA 的免疫反应，STING通路通过调节I型干扰素来参与固有免疫，同时引起较强的抗原特异性B细胞，T细胞反应。STING是miR-24可能的靶基因，但尚未有关于miR-24 通过STING信号通路作用于DNA疫苗免疫效应的研究报道。本课题在前期研究的基础上，从以下方面对miR-24通过STING信号通路作用于靶向防龋DNA疫苗免疫效应的机制进行了研究。 .本项目组使用新型电核转仪转染靶向防龋DNA疫苗质粒进入DC细胞，激活细胞的固有免疫，检测发现miR-24在靶向防龋DNA疫苗质粒激活的DC细胞固有免疫过程中表达下降。进而通过信息学分析、荧光素酶实验等，阐明miR-24调控STING表达的分子机制。完成了大部分研究目标，为进一步提高靶向防龋DNA疫苗的免疫反应水平提供新的有潜力的研究靶点。