卵巢浆液性囊腺肿瘤基因表达差异的研究

Ovary cancer in female gestational track is the first enemy for woman. Its' death-rate is over the death-rate of the cancer of cervical plus endometrium. There no outstanding advances in research on early diagnosis of ovary cancer for many years. To find new molecular maker become more important. In this study, the samples of ovarian serous cystadenocarcinoma and serous cystadenosarcoma are collected, then total RNA was isolated and reverse transcript to cDNA. Differential display PCR was performed by anchor primer and random primer. The 21 differential display bands in cystadenocarcinoma were found by PAGE electrophoresis. There are 3 bands higher expression in cystadenocarcinoma by dot blotting. One of the 3 bands is new sequences, the second one is refer to telomerase gene and the third one is known sequence by search NCBI. The telomerase sequence and the third band shown high expression in cystadenocarcinoma by dot blotting and in situ hybridization. Wu improved the technique of DD PCR by simplified the subtract hybridization and isotopic labeling. And we explored using 4 primers and anchor primer for co-amplification.The new sequence we found has been submit to GENEBANK. Three papers have been written.

用基因表达差异显示法分析良恶性和交蚧性卵巢浆液性囊腺肿瘤基因表达的差异，筛选与囊腺癌相关的基因片段，经克隆和测序后，进一步研究其表达和分布特点确定其与该肿瘤恶性度的关系探讨该肿瘤良恶性间在分子水平的差异，为进一步克隆全基因、研究表达产物与资助液性囊腺癌发生的关系、寻找早期诊断的标志基因和标志物及评价临床治疗效果奠定基础。