从Wnt/β-catenin/TCF信号通路研究热敏灸干预慢性萎缩性胃炎癌前病变的效应机制

Chronic atrophic gastritis(CAG) with gastric mucosal atrophy, inherent glands decreased or disappeared, intestinal metaplasia and atypical hyperplasia as the main symptoms, and its canceration rate is high, its pathogenesis closely associated with Wnt/β-catenin/TCF signaling pathway and acupuncture in the treatment of CAG has whole regulation advantage and moxibustion on heat sensitive acupoints has high sensitivity, which can make stimulate the Qigan easy, so that gas can easily go to disease and we can improve the clinical efficacy of moxibustion. In this study, the clinical scientific research as the basis, infrared imaging technology as heat sensitive point detection method, CAG rats in the "Zhongwan" point with heat sensitive moxibustion as the intervention measures, Wnt/β-catenin/TCF signaling pathway as the starting point , CAG rats model as observation object, using ELIAS, REAL time-PCR, WESTERN BLOT, immunohistochemical method and other technical means and through the multiple links and complex relation between Wnt 2, β-catenin, GSK-3β, TCF4, c-Myc, cyclin D1 and so on to Wnt signaling pathway to explore the possible mechanism of the efficacy action of heat sensitive moxibustion treated in the precancerous lesions of chronic atrophic gastritis, which is can provide some experimental basis for the clinical application of heat sensitive moxibustion treated in CAG

慢性萎缩性胃炎（CAG）主要病理特点是胃黏膜萎缩、固有腺体减少或消失，伴肠上皮化生、不典型增生等,癌变率高。其发病机制与Wnt/β-catenin/TCF信号通路密切相关，针灸治疗CAG具有整体调节优势，艾灸热敏穴具有高度敏感性，极易激发经气感传，气至病所，提高临床疗效。本研究以临床科研为依据，以红外成像为热敏穴检测技术，以热敏灸CAG大鼠“中脘”穴为干预措施，以Wnt/β-catenin/TCF信号通路为研究切入点，以CAG大鼠模型为观察对象，运用ELISA、Real time-PCR、Western blot、免疫组化等多种技术手段，从Wnt2、β-catenin 、GSK-3β、TCF4、c-Myc、cyclin D1等与Wnt信号通路多个环节的复杂关系中，探讨热敏灸对CAG癌前病变治疗作用的可能机制，为热敏灸治疗CAG的临床应用提供部分实验依据。

慢性萎缩性胃炎（CAG）主要病理特点是胃黏膜萎缩、固有腺体减少或消失，伴肠上皮化生、不典型增生等,癌变率高。其发病机制与Wnt/β-catenin/TCF信号通路密切相关，针灸治疗CAG具有整体调节优势，艾灸热敏穴具有高度敏感性，极易激发经气感传，气至病所，提高临床疗效。. 66只雄性SD大鼠随机分为空白组（12只）和造模组（54只）。空白组不予干预；造模组采用复合病因造模法造模12周，随机分为模型组（11只）、维酶素组（11只）、艾灸组（22只）。艾灸组悬灸“中脘”穴40 min，艾灸后按2 mL/kg/d灌服0.9%氯化钠溶液，根据悬灸过程中大鼠尾温变化分为热敏灸组和非热敏灸组；维酶素组等量灌服维酶素；空白组和模型组不予干预。每日1次，共28 d。实验结束后检测各组数据，显示热敏灸组大鼠Wnt2、β-catenin 、TCF4及c-Myc、cyclin D1、survinin等表达下降，恢复细胞增殖与凋亡平衡，调控大鼠胃黏膜形态，表明热敏灸能通过调控Wnt/β-catenin/TCF通路达到干预CAG癌前病变的效应机制。. 本项目研究成果初步揭示了灸疗热敏的生物学基础，对丰富和发展腧穴敏化理论具有一定的作用，证实了热敏灸对CAG大鼠胃黏膜形态学具有良好的干预调控作用，为热敏灸干预CAG的癌前病变临床应用提供了部分实验依据，进一步推动针灸防治CAG癌前病变的机制研究，并可促进热敏灸干预CAG在临床上的使用和推广，提高人民生活健康水平，具有十分重要的社会和经济意义。