基于金铂核壳结构功能化二硫化钼生物传感器的构建及其在肺癌标志物检测中的应用

Nanobiosensor has widely used in food industry, environmental monitoring, genetic analysis, health care and disease diagnosis because of its high sensitivity and excellent stability. It is important to design and construct detection system for microRNA or proteins detection with high sensitivity and selectivity because it has very important clinical significance for lung caner diagnosis, individualized treatment and prognosis. It should be noted that the properties of nanomaterials is greatly influence the detection performance of nanobiosensor. This project plans to synthesize different size, morphology and composition of gold-platinum core-shell nanoparticles-decorated molybdenum disulfide (MoS2) nanocomposites by changing the reaction conditions. The relationship between MoS2-based nanocomposites and nanobiosensor’s performance is system studied. The best catalytic performance and the most controllable MoS2-based nanocomposites have been selected to construct nanoprobe due to its unique advantages, such as high catalytic ability, high stability and easy functionalization. Combined with advantages of the colorimetric and electrochemical biosensors, such as simple and fast, a series of MoS2-based biosensors have been developed for lung biomarkers detection (e.g. microRNA and proteins). Our aim is to construct a universal detection strategy for 3-4 lung biomarkers detection with ultrahigh sensitivity, visualization and high-throughput. The MoS2-based detection platform is also used to detect lung biomarkers in serum samples, which offers a possible detection method for early screening and early diagnosis of lung cancer. It also proves that nanomaterials can be for the early diagnosis of cancer.

纳米生物传感器具有检测灵敏度高和稳定性好等特点，已广泛应用于食品工业、环境监控、基因分析、医疗保健和疾病诊断等诸多领域。设计、构筑针对microRNA或蛋白质等肺癌标志物高灵敏、特异性的检测体系对肺癌的早期诊断、个体化治疗、预后判断具有十分重要的临床指导意义。其中纳米材料自身的性质极大影响纳米生物传感器的检测性能。本项目拟调控合成大小、结构形貌和组成比例不同的金铂核壳纳米颗粒功能化二硫化钼复合材料，探索和阐明该纳米复合材料与传感器检测性能之间的关系。利用该纳米复合材料所具有的纳米酶特性，如催化活性高、稳定性好、易于功能化等，结合比色法和电化学技术快速、简单的优势，构建高性能生物传感器，实现对3-4种肺癌标志物超高灵敏、可视化和特异性的联合检测。将该检测策略用于血液中肺癌标志物的超高灵敏检测，为肺癌患者早期诊断的提供检测方法，也为纳米材料应用于癌症早期诊断提供数据和理论支持。

重大疾病的早期检测对于提升病人生存率具有至关重要的意义。本项目选取具有催化活性高、比表面积大等优异特性的二硫化钼基纳米复合材料为研究对象，系统研究了二硫化钼基纳米复合材料的模拟酶活性、催化活性，利用生物分子与二硫化钼基纳米材料间相互作用，构筑了一系列纳米生物探针，结合电化学、比色等分析技术与信号放大技术，构建了高性能生物传感平台，实现了microRNA-21、癌胚抗原等肺癌标志物高灵敏、特异性的检测。不仅如此，所构建的生物传感器还可以实现血清、细胞等实际样本中肺癌标志物的高灵敏检测，这为二维纳米材料在生物传感、疾病诊断等领域的应用提供研究思路和技术支撑。本项目的部分研究成果，已陆续在Biosensors and Bioelectronics、Trends in Analytical Chemistry、ACS Applied Materials & Interfaces、Nanoscale、Green Energy & Environment等国际SCI期刊上发表相关学术论文13篇，申请中国发明专利2件。