基于碳纳米管导电纳米材料的心肌组织工程研究

Engineered cardiac tissues (ECTs) showed geart prospect in the therapeutic application in myocardial infarction. Recently, considering the native electrical property of myocardium, electrionial conductive nano-scaffolds have been successfully used to fibracate ECTs in vitro, and carbon nanotubes(CNTs) have been at the forefront of nanotechnology due to their unique electrical and mechanical properties, which can promote the viability and proliferation of cardiomyocytes and enhance cardiomyocytes to obtain a more mature electrophysiological property. Here we constructed ECTs by CNTs composite scaffolds, and evaluate the contribution and influence of CNTs on the development of cardiomyocytes and 3D reconstruction of cardiac tissues,especialy the mechanism of CNTs on the proliferation, development and electrial mature of cardiomyocytes. Furthermore, we transplanted ECTs in rat infarcted myocardium, and investigate the structural, electrical and functional intergration between ECTs and infarcted myocardium,especially the role and the mechanism of CNTs in this process. Our research would make a further step on the therapeutic application of ECTs in myocardial infarction by using electrionial conductive nano-scaffolds, and establish the basis for the application of electrionial conductive nano-scaffolds in cardiac tissue engineering.

工程化心肌组织（ECTs）在心肌梗死治疗中具有重要应用前景，近年来，鉴于心肌组织独有的电生理特性，应用导电纳米材料作为细胞支架开展心肌体外再造研究取得明显进展。碳纳米管材料具有良好的力学性能和导电特性，前期研究表明，其具有促进大鼠未成熟心肌细胞增殖与成熟心肌细胞进一步发育的作用。为此，本项目拟利用基于碳纳米管的复合导电支架体外构建ECTs，并系统评价碳纳米管这一导电纳米材料对心肌细胞发育及心肌组织三维组装形成的影响规律，深入研究碳纳米管导电材料对心肌细胞增殖、发育与电生理相关的分子表达影响的规律和机制；在此基础上，将基于碳纳米管支架的ECTs移植到心梗部位，分别从结构、电生理和心功能三方面评价ECTs与宿主心肌组织的整合效果，以及碳纳米管在整合过程发挥的作用和机制。本研究将有望在基于导电纳米材料构建ECTs进行心梗修复方面取得重要突破，为新型导电纳米材料在心肌组织工程中的应用奠定基础。