新型珍珠粉荧光碳点的制备及其周围神经示踪的研究

Neural tracing technology is the one of the most commonly used method of neuroanatomy and nerve regeneration studies. It provides direct evaluation of nerve regeneration by comparing with electrophysiological and behavioral examinations, and reflects the degree of nerve function recovery. There are many disadvantages in the traditional neural tracer techniques, such as weak fluorescence signal, photobleaching easily and low sensitivity. In recent years, the discovery of carbon dots (CDs) brings innovative spot and hope to the traditional neural tracer technology. As a new type of nano material, the particle size of CDs is less than 10nm, which has excellent properties including photoluminescence, anti-photobleaching, chemical medication and so on. We have found a new method for the preparation of CDs (enzyme digestion), and used this method to obtain the innovative pearl powder fluorescent CDs(PFCDs). The EDC/NHS method was applied to combine the amino group of PFCDs and the carboxyl group of the cholera toxin CTB together. Based on the optical properties of PFCDs and the.binding properties of neural cell surface GM1 to CTB, it is designed to coupling the CTB-PFCDs, which presents to be an economical, efficient method for tracing the peripheral nerve. This study not only lays the experimental foundation for the development of a new generation of PFCDs related nerve tracer, but also broadens the mind in this field.

神经示踪技术是神经解剖学和神经再生研究中最常用的方法之一，较神经电生理和行为学检查可更直观地评价神经再生水平，并能反映神经功能恢复的程度。传统神经示踪技术存在荧光信号弱、易光漂白、灵敏度姣低等不足。今年，碳点(CDs)的出现对传统神经示踪技术的创新研究带来新的亮点与希望。 CDs作为新型纳米材料,其粒径一般在10nm 以下，具有优良的光致发光性能、抗光漂白、易于化学修饰等。我们研究发现了新的制备碳点的方法（酶切法），并利用此法成功制备出新型珍珠粉荧光碳点（PFCDs）。利用EDC/NHS方法将PFCDs的氨基与霍乱毒素CTB的羧基相互连接形成偶联体。利用PFCDs光学性质和CTB与神经细胞膜表面 GM1 神经节苷脂结合的靶向特性，设计了CTB-PFCDs偶联体的更直接而更经济的周围神经逆行示踪新方法。本研究为新一代PFCDs相关的神经示踪剂的研发奠定了实验基础，拓展了新的思路。

神经示踪技术是神经解剖学和神经再生研究中最常用的方法之一，较神经电生理和行 为学检查可更直观地评价神经再生水平，并能反映神经功能恢复的程度。传统神经示踪技 术存在荧光信号弱、易光漂白、灵敏度姣低等不足。今年，碳点(CDs)的出现对传统神经 示踪技术的创新研究带来新的亮点与希望。 CDs作为新型纳米材料,其粒径一般在10nm 以 下，具有优良的光致发光性能、抗光漂白、易于化学修饰等。我们研究发现了新的制备碳 点的方法(酶切法)，并利用此法成功制备出新型珍珠粉荧光碳点(PFCDs)。利用EDC/N HS方法将PFCDs的氨基与霍乱毒素CTB的羧基相互连接形成偶联体。利用PFCDs光学性质和C TB与神经细胞膜表面 GM1 神经节苷脂结合的靶向特性，设计了CTB-PFCDs偶联体的更直接 而更经济的周围神经逆行示踪新方法。本研究为新一代PFCDs相关的神经示踪剂的研发奠 定了实验基础，拓展了新的思路。