ABPPk通过调控lncRNA094268/miR-221-3p/Cntn1轴促进施万细胞成髓鞘的作用及机制研究

Peripheral nerve injury repair is a difficult problem in medical field, and development of new drugs is necessary for nerve regeneration in clinic. ABPPk is an effective component of traditional Chinese medicine for purifying Achyranthes bidentata polypeptides and has been proven to have good neuroprotective effects. Preliminary research shows that ABPPk could effectively promote myelination of schwann cells while transcriptome sequencing suggests that Cntn1 plays a role in this process. The ceRNA regulatory network analysis revealed that lncRNA 94268 interacts with miR-221-3p, and miR-221-3p is associated with Cntn1. So we propose the hypothesis that in peripheral nerve regeneration after the addition of ABPPk, lncRNA 94268 acts as a ceRNA of miR-221-3p to promote the up-regulation of Cntn1 to promote myelination. Based on the previous research, this study aims to clarify the role of ABPPk in promoting the myelination of Schwann cells, and then to study the mechanism of ceRNA network regulation to clarify its mechanism. Furthermore, we will determine the interaction and function of the ceRNA. Finally, we will reveal the effect on peripheral nerve regeneration. This project will help us to reveal the key scientific issues of a close relationship between traditional Chinese medicine and peripheral nerve regeneration, and provide a foundation for the exploration of treatment targets on peripheral nerve injury repair.

周围神经损伤修复是医学界的难题，需开发促神经再生的新药以满足临床需求。ABPPk作为牛膝多肽提纯的中药有效成分，证实有很好的神经元保护作用。前期研究表明ABPPk有效促进施万细胞成髓鞘，同时全转录组测序提示Cntn1在此过程中发挥作用。ceRNA调控网络分析发现lncRNA094268与miR-221-3p相互作用，miR-221-3p又与Cntn1相互关联，基于此我们推测lncRNA094268作为miR-221-3p的ceRNA促进Cntn1上调，ABPPk由此机制促进髓鞘形成。本课题拟在前期研究基础上明确ABPPk促进施万细胞成髓鞘的作用；接着对ceRNA网络调控机制深入研究，阐明其作用机制；在此基础上确定ceRNA网络中三者的相互作用及功能；最后在整体水平揭示其对周围神经再生的影响。本项目将有助于阐释传统中药与周围神经再生的关键科学问题，为周围神经损伤治疗靶点的探寻提供依据。

周围神经损伤修复是医学界的难题，需开发促神经再生的新药以满足临床需求。ABPPk作为牛膝多肽提纯的中药有效成分，证实有很好的神经元保护作用。本研究发现ABPPk可有效促进周围神经损伤修复及施万细胞成髓鞘，同时全转录组测序提示Cntn1在此过程中发挥作用。进一步研究其ceRNA调控机制，揭示了lncRNA 094268/miR-221-3p/Cntn1对施万细胞功能及成髓鞘的作用，为将Cntn1作为周围神经损伤修复的新靶点提供实验依据。本研究对ABPPk的功能和作用机制进行了进一步的探究，阐明这一机制有利于发现新的治疗靶点，也有利于发现新的治疗途径，以满足临床的迫切要求，具有潜在的科学和临床价值。