仿生导电高分子的可控微纳米组装及循环肿瘤细胞的高效率捕获及传感

Liquid biopsy, which could detect circulating tumor cells (CTC) in a small amount of blood, possesses several advantages superior to the traditional biopsy, such as rapid diagnosis, easy operation, painlessness and noninvasiveness. If a high efficient capture and isolation of CTC could be integrated with a sensitive on-line detecting of CTC onto one platform, the liquid biopsy would work more efficiently but at an easy rate, which would significantly enhance our capability to detect early tumor and efficiency to treat cancer. . We propose here to develop a new template approach to realize a controllable micro- and nano-assembly of biomimicking PEDOT conducting polymers. We will further adopt this approach to fabricate a series of biomimicking PEDOT conducting polymers with various micro- and nano-structures and functionalities. By finely tuning the nanostructures and functionalities of biomimicking conducting polymers, we aim at a conducting platform with not only a high efficient and selective isolation of CTC but also a sensitive on-line impedance detection of CTC. Hopefully, the micro- and nano platform integrating both capture and sensing of CTC could illuminate a potential path for an efficient but low cost detection of early tumor, and pave a material foundation for the design of liquid biopsy sensors.

相对于组织活检，通过从微量血中捕获CTC而实现的液体活检技术，具有快速、简便、无痛苦、无创伤等优势。如能在CTC有效捕获富集的基础上实现其即时传感检测，将使得液体活检技术更有效率和更廉价，进而对于实现癌症早期筛查、疗效效果评估具有重要意义。因此，本项目拟发展一种原位生长的仿生导电高分子微纳米传感材料的制备方法，实现仿生PEDOT材料的可控微纳米组装，并系统研究其表面结构及组成与CTC捕获效率、选择性及电化学传感的关系，在达成高效率高选择性捕获电化学即时传感的基础上初步探索其在CTC传感芯片上的应用，为癌症早期检测提供新的研究思路，并为最终制备液体活检生物电子器件奠定坚实的材料基础。

通过本项目三年的持续研究，发展了针对多种功能化PEDOT材料的可控微纳米组装技术，实现了具高长径比纳米管形貌的功能化PEDOT薄膜无模板组装及其形貌参数的可调控，获得了兼具高电化学性能及靶向细胞结合性能的敏感材料；同时增加了基于仿生PEDOT材料的电化学晶体管研究工作，制得了有机电化学晶体管传感器，实现了仿生功能化PEDOT材料在有机电化学晶体管上的集成，并研究了器件的物理性能、抗非特异性蛋白粘附性能及靶向细胞结合性能，实现了在高抗非特异性粘附背景下对靶向细胞的选择性结合。该研究结果为实现全血中痕量CTC的即捕获即传感打下了材料与器件的基础，有望为癌症液体活检提出一有潜力的技术途径，在癌症早期诊断领域有着较大的应用潜力。