用ICAM-1反义RNA治疗肾移植排斥反应的实验研究
基本信息
结项摘要
本研究首先构建了ICAM-1反义DNA表达载体，证明ICAM-1反义DNA可明显抑制细胞ICAM-1表达。然后建立了腺病毒载体转移系统，证明该系统具有高转染率、高表达特点，介导ICAM-1反义DNA体外可明显抑制细胞ICAM-1表达。最后制备了大鼠单肾缺血再灌注模型，在肾缺血前6小时经尾静脉注射含ICAM-1反义DNA的腺病毒，结果证明ICAM-1反义DNA体内也明显抑制肾脏细胞ICAM-1表达，并减少肾间质中性粒细胞浸润，减轻肾脏组织病理损伤，对肾功能有明显保护作用。肾缺血再灌注损伤腺病毒ICAM-1反义NDA注射大鼠血浆BUN为19.25±3.70mmol/L，Cr为62.0±9.55μmol/L,明显低于生理盐水对照组（BUN为79.90±12.35mmol/L,Cr为272±39.40μmol/L）而正常大鼠BUN为8.15±1.10mmol/L,Cr为28.5±4.60μmol/L.
项目摘要
项目成果
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数据更新时间：2023-05-31
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