基于反向胶体晶体和光子带隙的均匀孔径高效智能膜色谱材料制备研究

In the field of separation and purification of biomacromolecules, more and more attention has been paid to membrane chromatography technique, because it integrates the advantages of HPLC and membrane separation technology. However, there are some shortcomings in membrane chromatography material (MCM) so far, such as the heterogeneity of bore diameter, the unsatisfactory porosity and specific surface area, and the lack of intelligent response to the amount of adsorbates or the contaminants, and so on. Accordingly, how to improve the property of the MCM becomes the issues of concern. In the project, we make full uses of the advantages of inverse colloid crystal (ICC), including the uniform aperture distribution, the high porosity, and the photonic bandgap which can promote effectively the optical sensing characteristics to the amount of the adsorbates or the contaminants, and make innovations to the material preparation, structure design, and performance optimization, in order to establish a systematic preparation method based on the high performance inverse colloid crystal membrane chromatography material (ICCMCM), which possessing the structural controllability, intelligent responsiveness, and applicability for affinity chromatography, hydrophobic chromatography, ion exchange chromatography, multistage chromatography separation, etc. To realize the goal, we develop the design of colloidal crystal template and molecular structure to control the microstructure and performance of MCM, and research the chemical and physical properties of RCCMCM and optical sensing characteristics, so that explore the application of ICCMCM in high efficient intelligent separation and purification of biomacromolecules.

膜色谱技术因融合了液相色谱和膜分离技术二者之长，在生物大分子的分离纯化中越来越受到人们的重视，目前的膜色谱材料存在孔径不均匀、孔隙率和比表面积有待提高、膜对于吸附物或污染物含量缺少智能响应等缺点，因此如何进一步提高膜色谱材料的性能成为一个备受关注的问题。本课题旨在利用反向胶体晶体的均匀孔径和高孔隙率、及其光子带隙对吸附物或污染物含量的光学传感特性，通过对膜色谱材料制备、结构设计和性能的创新，建立起具有结构可控，对吸附物或污染物含量智能响应，适合亲和、疏水、离子交换和多级色谱分离等应用需要的高性能反向胶体晶体膜色谱材料制备的系统方法。利用胶体晶体模板和分子结构设计实现对膜色谱材料微结构和性能的控制，研究所制备的反向胶体晶体膜色谱材料的物理化学性质和基于光子带隙的光学传感特性，探索其在生物大分子高效智能分离纯化等方面的应用。

膜色谱技术因融合了液相色谱和膜分离技术二者之长，在生物大分子的分离纯化中越来越受到人们的重视，目前的膜色谱材料存在孔径不均匀、孔隙率和比表面积有待提高、膜对于吸附物或污染物含量缺少智能响应等缺点，因此如何进一步提高膜色谱材料的性能成为一个备受关注的问题。本课题利用反向胶体晶体的均匀孔径和高孔隙率、及其光子带隙对吸附物或污染物含量的光学传感特性，通过对膜色谱材料制备、结构设计和性能的创新，建立了具有结构可控，对吸附物或污染物含量智能响应，适合亲和、疏水、离子交换和多级色谱分离等应用需要的高性能反向胶体晶体膜色谱材料制备的系统方法。利用胶体晶体模板和分子结构设计实现了对膜色谱材料微结构和性能的控制，研究了所制备的反向胶体晶体膜色谱材料的物理化学性质和基于光子带隙的光学传感特性，探索了其在生物大分子高效智能分离纯化等方面的应用。