移植IGF-1基因工程雪旺细胞修复脊髓脱鞘损伤的研究

One important therapeutic goal during CNS injury from trauma or demyelinating diseases such as multiple sclerosis is to develop methods to promote remyelination. To investigate the possibility that populations of purified Schwann cells genetically modified to secrete insulin-like growth factor-1(IGF-1) can be used to promote remyelination of demyelinating CNS lesions, we transplanted a variety of preparations of purified Schwann cells genetically modified to secrete IGF-1 into demyelinating lesions in the spinal cords. The amounts of IGF-1 secreted were 19 and 6ng/24h per 106 cells for infected and normal SCs, repectively. Biological activity of the secreted IGF-1 was confirmed by MTT bioassay. The use of Hoechst prelabeled SCs demonstrated that trails were maintained at least for 6 weeks. More expressions of mRNA and protein of MBP、PLP and CNP in the lesion areas were observed in the infected vs. normal SCs. Compared with normal SCs, transplantation of infected SCs into the demyelinated rodent spinal cord results in anatomically defined myelination and near normal conduction properties of the remyelinated axons ensue

脊髓髓鞘脱失是许多非免疫和免疫性损伤的中同病理过程，目前尚无有效的修复手段。本实验拟运用逆转录病毒载体pLNX为载体，将类胰岛素生长因子（IGF-1）转导致新生大鼠雪旺赴⊿C），再将转染IGF-1基因的SC移植到局灶性脱髓鞘损伤的动物脊髓内，观察IGF-1基蛐奘蔚腟C对中枢脱髓鞘损伤的修复作用，为中枢神经损伤的修复探索新途径。