磁性Fe3O4/PS基纳米材料的制备及其调控脂肪酶催化活性研究

Our research is focusing on the synthesis of magnetic Fe3O4/PS based nanocomposites for activating lipases. Magnetic Fe3O4/PS nanocomposites with controllable sizes and curvature will be synthesized by an emulsion polymerization. A technical scheme for surface modification of Fe3O4/PS nanocomposites will be created to synthesize magnetic Fe3O4/PS based nanocomposites (Fe3O4/PS/PEG, Fe3O4/PS/PVA, Fe3O4/PS/PAA, Fe3O4/PS/SiO2) with controllable curvature and hydrophilic/hydrophobic properties. Importantly, an activating lipases strategy basing on the excitation arousing from the different surface properties of magnetic Fe3O4/PS based nanocomposites will be established. The effect of the magnetic Fe3O4/PS based nanocomposites on the structure and catalysis of lipases will be studied for searching magnetic nanoexcitants (magnetic Fe3O4/PS based nanocomposites). Also, the effect of magnetic nanoexcitants on the improvement of catalysis efficiency of lipases will be investigated systematically to obtain scientific basis on the excitation of nanomaterials. This project can solve key technical problems on synthesis of Fe3O4/PS based nanocomposites and activating oil/water interfacial activation of lipases, which not only extends the application of nanomaterials in the field of bio-catalysis, but also provides scientific basis for the study of activating enzymes.

本项目围绕"磁性Fe3O4/PS基纳米材料的制备及其调控脂肪酶催化活性研究"拟开展以下研究：（1）采用乳液聚合法制备大小和表面曲率可控的磁性Fe3O4/PS纳米材料；（2）研究磁性Fe3O4/PS纳米材料的表面修饰，构建表面曲率和亲/疏水性可控的磁性Fe3O4/PS基纳米材料；（3）建立利用磁性Fe3O4/PS基纳米材料的表面曲率和亲/疏水性调控脂肪酶催化活性的方法，研究磁性Fe3O4/PS基纳米材料对固定在其表面脂肪酶蛋白结构和功能的影响，筛选磁性纳米活化剂（能提高脂肪酶催化活性的磁性Fe3O4/PS基纳米材料）；（4）探讨磁性纳米活化剂调控脂肪酶催化活性的构效关系，获取纳米材料调控脂肪酶催化活性的科学依据。本项目研究可解决磁性Fe3O4/PS基纳米材料的制备和脂肪酶油/水界面催化活化的关键技术问题，不仅能拓展纳米材料在生物催化领域中的应用，而且为其它酶制剂的活化研究提供有用信息。

本项目围绕“磁性Fe3O4/PS基纳米材料的制备及其调控脂肪酶催化活性研究”开展以下研究：（1）采用乳液聚合法制备出油/水双分散的磁性Fe3O4/PS纳米粒子和海胆状Fe3O4/PS纳米粒子；（2）研究磁性Fe3O4/PS纳米材料的表面修饰方法，制备出表面曲率和亲/疏水性可控的Fe3O4/PS/PEG、Fe3O4/PS/PVA、Fe3O4/PS/PAA、Fe3O4/PS/SiO2纳米材料；（3）建立利用磁性Fe3O4/PS基纳米材料的表面曲率和亲/疏水性调控脂肪酶催化活性的方法，研究磁性Fe3O4/PS基载体材料的尺寸、稳定性、表明亲疏水性对固定在其表面脂肪酶蛋白结构和功能的影响，筛选磁性纳米活化剂（磁性Fe3O4@PVBC纳米粒子）；（4）探讨磁性纳米活化剂调控脂肪酶催化活性的构效关系，获取纳米材料调控脂肪酶催化活性的科学依据。本项目研究可解决磁性Fe3O4/PS基纳米材料的制备和脂肪酶油/水界面催化活化的关键技术问题，不仅能拓展纳米材料在生物催化领域中的应用，而且为其它酶制剂的活化研究提供有用信息。