药性影响血液多元参数与机体形态结构的相关研究

The narure of Chinese matreia medica is belong to the core content of its basic research , is the important bridge cohesion of Chinese materia medica and Traditional Chinese Medicine therefor is the important scientific issues of modernization of Traditional Chinese Medicine. Based on the growing consensus that signal conditioning and body metabolism is closely related to the nature of Chinese Materia Medica, this application is based "the combined impact of regulatory signals and metabolism by the nature of Chinese Materia Medica , which cause the body's patterned reaction of ordered structure " for the study hypothesis and intends to proposed using NMR spectroscopy to determine serum metabolomic and LC-MS to determine serum neurotransmitters and hormones to explore the link among dynamic changes of serum metabolomic, neurotransmitters, hormones and the morphology functionin fluenced by the nature of Chinese Materia Medica which was on the basis that initial results of the early issues "the study on body organs and tissues related to the nature of Chinese Materia Medica " has been achieved, which may lay the foundation of experimental basis necessary for the future to solve integration problem of the nature of Chinese Materia Medica - chemical ingredients - pharmacological effects.

中药药性是中药基础研究的核心内容，是衔接中药学与中医学的重要桥梁，因而是中医药现代化的重大科学问题。在药性与机体信号调节和物质代谢密切相关日趋获得共识基础上，本申请以"中药药性组合式影响调控信号和物质代谢，从而引起机体有序结构的模式化反应"为研究假说，拟在前期课题"中药性味归经与机体器官组织相关性研究"已取得阶段性成果的基础上，采用核磁共振光谱测定血清代谢物组、液质联用测定血清神经递质及激素，探索形态结构机能与血清代谢物组、神经递质及激素在中药药性影响下动态变化的联系，为今后解决中药药性-化学成份-药理效应整合问题奠定必要的实验基础。

本项目从机体器官组织机能、神经内分泌免疫系统及机体内源性代谢物的变化层面上分析中药药性作用机体整体的机制，探求中药药性与机体整体二者之间的内在联系。为尝试阐述中药药性作用生物学机制提供实验基础。已完成所有计划的分别给予40味中药即刻、1小时、2小时、4小时后大鼠核.磁成像、采血测定激素及神经递质、核磁光谱测定血液代谢物组工作。研究结果显示：1、药性有一定的形态学基础。2、药性中的热性、归肾经、归三焦经，器官组织中的脑、肾脏、睾丸，神经递质及激素中的β-雌二醇、多巴胺，血液代谢物中的肌酐、酪氨酸有比较强的相关性，值得进一步探索研究。3、药性对机体不同参数的作用机制是不同的，各参数的参与程度是不同的。4、药性的作用时间可能由药性的不同成分作用特点所决定，结合各药性不同参与程度或可用于区分不同药性。5、本课题研究结果一定程度上证实了系统论整体不等于部分简单相加的观点。. 共发表相关论文6篇，其中sci期刊1篇。