调控鹅掌楸叶形发育基因的发现与功能鉴定

Leaf shapes are the most common phenotypic traits in plants. However, the mechanism underlying leaf shape development keeps unknpwn. Due to its peculiar leaf shapes, obvious interspecific difference, and remarkable segregation among interspecific F1 populations, Liriodendron are ideal plants for exploring the mechanism underlying leaf shape development. This project aims at discovering and characterizing genes that regulate leaf shape development in Liriodendron. The research ideas and strategies are as follows: Transcriptome data of various tissues relating to leaf deveopment will be used to mine differential expression genes (DEGs). The full-length sequence of genes relating to leaf shape development will be isolated through RACE technique. The function validation of isolated genes will be executed via qRT-PCR and gene transfer technology. Furthermore, those novel genes will be verified phenotypically and genotypically in a interspecific F1 population. This findings may have potential application value in future tree breeding

叶形是植物最常见的表型性状，但关于叶形发育的调控机理目前仍不清楚。鹅掌楸叶形奇特，且种间差异大、杂种子代叶形分化明显，因而是研究叶形发育及调控机理的较理想材料。本项目利用RNA-seq技术进行转录组测序，通过比较不同叶形、不同发育阶段的转录组信息，挖掘鹅掌楸叶形发育相关基因。利用RACE技术克隆基因全长，并通过qRT-PCR及遗传转化技术进行基因功能分析。同时，选择杂种子代叶形分化大的群体从表型、基因表达两方面进行验证，在此基础上，探索鹅掌楸叶形发育的遗传调控机制。

叶形是植物最常见的表型性状，但关于叶形发育的调控机理目前仍不清楚。鹅掌楸叶形奇特，且种间差异大，因而是研究叶形发育及调控机理的较理想材料。本项目在系统观察鹅掌楸叶形发育过程基础上，采集不同发育时期的叶组织进行RNA-seq测序，结合鹅掌楸基因组信息与生物信息学预测结果，筛选出32个与鹅掌楸叶形发育相关候选基因。利用RACE技术克隆候选基因全长，并根据组织表达谱及亚细胞定位结果进一步筛选叶形发育相关基因。随后，构建超表达载体遗传转化拟南芥对目的基因的功能进行分析，最终，挖掘出7个鹅掌楸叶形发育相关基因，同时，还从基因调控网略、miRNA-lncRNA-TF调控两个层面初步揭示了鹅掌楸叶形发育的分子机制。本项目研究结果对于了解植物叶形态建成机制及叶形发育调控有理论与应用价值。