基于多重可控刺激响应型生物电催化构建生物分子逻辑门及其网络体系

The development of effective and convenient method and model for the construction of biomolecular logic gates and their network systems based on multiple controllable stimuli-responsive bioelectrocatalysis, could explore new ideas for multiple stumuli-responsive electrochemical biosensors. It also could provide the diagnosis of the disease and may solve some biomedical problems. This project intends to adopt simple, efficient, different assembly techniques and various stimuli-responsive materials to build biomolecular film electrodes, which have stimuli-responsive charateristic for the multiple and complex environmental stimuli. We can choose suitable materials and assembly conditions to regulate the characteristics of the films, especially its permeability to the components of the system. By applying different stimuli signals, the active/inactive enzyme logic gates and their networks could be realized. This work will combine the different kinds (single thin film, multiple films(layer-by-layer assembly), binary and multiple architecture) of stimuli-responsive films with biomolecular logic gate network, and propose a new idea to build multiple stimuli-responsive and active/inactive bioelectrocatalysis biomolecular logic network systems based on a combination of above two points.

发展简便有效的方法和模型构建多重可控刺激响应型生物电催化的生物分子逻辑门及其网络，可为发展基于多种酶反应的多重刺激响应型电化学生物传感器和基于逻辑门的生物电子器件开拓新的思路，为疾病的诊断以及解决某些生物医学问题提供可能。本项目拟采用简便快捷的不同薄膜制备方法和各种不同的刺激响应型材料来构筑对外界多重复杂环境刺激具有刺激响应性的生物分子薄膜电极，可以通过选择合适的成膜材料与组装条件来调控薄膜的性质，特别是其对溶液组分的通透性。通过施加不同的多重信号的刺激，实现激活/去激活生物电催化的生物分子逻辑门及其网络。该课题拟将多重刺激响应型各种（单层、多层(层层组装)、二元和多元结构）薄膜对溶液渗透组分的通透性与生物分子逻辑门网络相结合，在二者的结合点上提出了基于多重可控刺激响应型生物电催化构建生物逻辑门网络体系的新思路，具有新意。

发展了简便有效的方法和模型构建多重可控刺激响应型生物电催化的生物分子逻辑门及其网络，为发展基于多种酶反应的多重刺激响应型电化学生物传感器和基于逻辑门的生物电子器件开拓新的思路，为解决某些生物医学问题提供可能。采用了简便快捷的不同薄膜制备方法和各种不同的刺激响应型材料来构筑对外界多重复杂环境刺激具有刺激响应性的生物分子薄膜电极，通过选择合适的成膜材料与组装条件来调控薄膜的性质。通过施加不同的多重信号的刺激，实现激活/ 去激活生物电催化的生物分子逻辑门及其网络。该课题将多重刺激响应型薄膜对溶液渗透组分的通透性与生物分子逻辑门网络相结合，在二者的结合点上提出了基于多重可控刺激响应型生物电催化构建生物逻辑门网络体系的新思路，为开发新型的可调控的电化学生物传感器提供重要基础，并为多功能逻辑计算的发展奠定了理论基础。