可卡因对胎儿大脑发育的影响及其机理研究

Many researches related to cocaine reported that one of the main toxic targets of cocaine was dopaminergic system. The damage of dopaminergic system, which can regulate the growth and differentiation of neuron and control learning activity, will lead to the disorder of brain structure and function. We built up murine models who exposed to different dose cocaine in uterus in order to study the effects of prenatal cocaine exposure on mothers and offspring from the aspects of morphology, biochemistry and praxiology. We found that high dose prenatal cocaine exposure resulted in the death or the abortion of mothers. Low dose prenatal cocaine exposure lead to mother malnutrition and abnormal placentodevelopment. In addition, the content of dopaminergic neurotransmitter in cocaine fetus brains exited significant difference compared with control. Middling dose prenatal cocaine exposure caused the decent of offspring' brain weight and body weight, the abnormal function of dopaminergic system and structure of cortex and damaged their space distinguish abilities. These results above offered the neurobiology and morphology evidence to uncover the mechanism of cocaine toxicity which is important to improve the qualities of population.

本课题采用多巴胺D1和DAT受体基因剔除鼠，应用PCR、免疫组化、微透析、HPLC等方法并结合定量分析技术，观察母鼠可卡因中毒引起胎儿大脑发育异常的各项生理学、形态学和神经生化学指标，研究多巴胺D1和DAT受体在该过程中的调节作用及其可塑性和遗传性特征，为沂究煽ㄒ蛑卸净硖峁┮谰荨１究铺舛杂诳煽ㄒ虺神缘脑し兰坝派庞灿兄匾庖濉