脂联素及其受体对蛋鸡下丘脑-垂体-性腺轴的调控作用及分子机制

The hypothalamic-pituitary-gonadal(HPG)axis plays an important role in animal reproductive regulation. Researches regarding to factors and mechanisms regulating HPG axis function have become a hot topic in area of reproductive endocrinology. Recently, it has been evidenced that adiponectin(AdipoQ) and its receptors (AdipoRs) play an important part in regulating animal reproduction and embryo growth. However, most of the studies mainly focused on mammals, while the biological function and mechanism of AdipoQ and AdipoRs in regulating poultry reproductive performance remain unclear. In this project, layers will be taken as experimental animal, and in vivo and in vitra experiments will be carried out to investigate the distribution and localization of AdipoQ and AdipoRs in HPG axis. The morphology relationship between AdipoRs and hypothalamic GnRH neures, and the potential signaling pathways of AdipoQ regulating HPG axis will be explored by methods of immunohistochemistry, immunofluorescence and lase confocal microscopy, qRT-PCR, Western-blotting, histocyte culture, and intraventricular injection. The results gained from the project will elucidate the functional targets and mechanism of AdipoQ regulating HPG axis, which may lay the foundation for perfecting the theory of reproductive regulation in poultry.

下丘脑-垂体-性腺（HPG）轴在动物生殖调控扮演重要角色，研究调控HPG轴功能的因素及机制已成为生殖内分泌学领域的热点。近年来的研究发现，脂联素（AdipoQ）及其受体（AdipoRs）在调控动物生殖及胚胎生长发育方面起重要作用，这些研究大都集中在哺乳动物上，而AdipoQ及AdipoRs调控家禽生殖机能的生物学功能及其作用机制尚不明确。本项目以蛋鸡为试验材料，拟通过免疫组化、免疫荧光及激光共聚焦显微镜技术、qRT-PCR、Western-blotting、组织细胞培养、脑室注射等在体、离体试验系统研究AdipoQ及AdipoRs在蛋鸡HPG轴上的分布定位以及其与下丘脑GnRH神经元之间的形态学关系，同时探索AdipoQ及AdipoRs对HPG轴的调控作用和潜在的信号通路，阐明其作用靶点和调控机制，为完善家禽的生殖调控理论奠定基础。

为解析脂联素（AdipoQ）及受体（AdipoRs）调控家禽生殖机能分子机制，本项目以蛋鸡为试验材料，利用免疫组化、免疫荧光及激光共聚焦显微镜技术、qRT-PCR、Western-blotting、组织细胞培养、基因过表达或干扰等技术，结合在体、离体试验，系统研究了AdipoQ及AdipoRs在蛋鸡HPG轴上的分布定位以及其与下丘脑GnRH神经元之间的形态学关系；分析了产蛋周期的不同阶段 AdipoQ 及 AdipoRs 表达与 HPG 轴激素及其受体表达的关联性以及AdipoQ 过表达或干扰及AdipoRs 激动剂（AdipoRon）处理与蛋鸡 HPG 轴相关激素分泌和受体表达的关系；探讨了AdipoRs 过表达或干扰以及与AdipoRon 共处理对蛋鸡 HPG 轴激素分泌和受体表达以及潜在信号通路的影响。通过这些研究，揭示了 AdipoQ 及 AdipoRs 在蛋鸡 HPG 轴上的分布定位规律，阐明了其对蛋鸡HPG 轴的作用机制和信号途径，为完善家禽的生殖调控理论奠定了基础。