MjTTL8蛋白在爪哇根结线虫寄生番茄过程中的分子机制研究

Meloidogyne javanica is one of an important plant parasitic nematodes, which can infect a variety of important crops, and cause serious economic losses. In order to develop better policy to control root-knot nematode, the molecular mechanism of interaction between the host plant and root-knot nematodes needs to be further understood. The effectors secreted by root-knot nematode play a key role in the interaction between root-knot nematode and plant. In this project, one effector named MjTTL8 cloned from M. javanica, would be used to further study. The main purposes of this project are: to analyze the spatial and development expression and the secreted characteristics using RT-qPCR, in suit hybridization and immunofluorescence; to clarify the interaction between MjTTL8 and tomato using the yeast two-hybrid, bimolecular fluorescence complementary and Co-IP; to investigate the role of this protein in the process of root-knot nematode parasitism using RNAi; to analyze the interaction key site of the MjTTL8 using mutation. This project is likely to illustrate the expression profile, the target protein and the key site of MjTTL8. It may help us to understand the role of MjTTL8 in the root-knot nematode infection.

爪哇根结线虫是世界范围内重要的植物病原线虫，能侵染多种重要的农作物，并造成严重的经济损失。为了更好地防治根结线虫，我们需要增强对根结线虫与寄主植物之间相互作用分子机制的认识。根结线虫分泌的效应蛋白在根结线虫与寄主的相互作用中发挥关键的作用。本项目以本课题组从爪哇根结线虫中克隆得到的效应蛋白MjTTL8作为靶标，该蛋白含有N端信号肽，且编码该蛋白的基因在根结线虫食道腺特异表达。本项目拟通过龄期表达分析、组织表达特异性分析、分泌特性研究、酵母双杂交、荧光双分子互补、RNAi、蛋白作用位点分析等方法研究该蛋白在爪哇根结线虫寄生番茄过程中的分子作用机制。本项目可期望获得与爪哇根结线虫MjTTL8的表达特性、与MjTTL8相互作用的寄主靶蛋白、MjTTL8的关键作用位点以及了解MjTTL8在根结线虫寄生过程中的作用。

爪哇根结线虫是世界范围内重要的植物病原线虫，能侵染多种重要的农作物，并造成严重的经济损失。为了更好地防治根结线虫，我们需要增强对根结线虫与寄主植物之间相互作用分子机制的认识。根结线虫分泌的效应蛋白在根结线虫与寄主的相互作用中发挥关键的作用。本项目以本课题组从爪哇根结线虫中克隆得到的效应蛋白MjTTL8作为靶标，该蛋白含有N端信号肽，且编码该蛋白的基因在根结线虫食道腺特异表达。本项目拟通过龄期表达分析、组织表达特异性分析、分泌特性研究、酵母双杂交、荧光双分子互补、RNAi、蛋白作用位点分析等方法研究该蛋白在爪哇根结线虫寄生番茄过程中的分子作用机制。本项目可期望获得与爪哇根结线虫MjTTL8的表达特性、与jTTL8相互作用的寄主靶蛋白、MjTTL8的关键作用位点以及了解MjTTL8在根结线虫寄生过程中的作用。