基于高光谱成像技术与甲烷检测的小麦非生物复合胁迫交叉响应机理研究

Wheat is the main grain crop in China, which plays an important role in the development of the national economy. However, the abiotic stresses such as ultraviolet radiation, high temperature, low temperature, physical wounding and water stress, etc. formed under natural conditions have become important factors to restrict the yield and quality of the wheat. Due to the problems that the fuzzy of cross reaction mechanism of multiple abiotic stresses in wheat and tedious and hysteretic of the traditional detection methods, we plan to adopt hyperspectral imaging technique and infrared absorption spectroscopy technique for the research of cross reaction mechanism of multiple abiotic stresses in wheat. The analysis of the characteristics of the wheat, the exploration of endogenous methane production and release mechanism, etc. will be carried out under multiple abiotic stresses. Finally, the purpose of revealing cross reaction mechanism of multiple abiotic stresses in wheat and the actual prediction ability of multiple abiotic stresses will be realized.. Following the analysis of cross reaction mechanism of multiple abiotic stresses in wheat, the multiple abiotic stresses prediction model based on methane detection will be established, which can provide theoretical basis and technical support to ensure the healthy and efficient plant growth. The research not only has high scientific research value, but also has important realistic meaning.

小麦是我国的主要粮食作物，在国民经济发展中具有举足轻重的地位。然而，紫外辐射、高温、低温、物理损伤、渗透胁迫等自然条件下形成的非生物胁迫，逐渐成为制约小麦产量和品质的重要因素。针对当前非生物复合胁迫条件下小麦交叉响应机理尚未阐明，以及传统检测手段步骤繁琐、实时性差等问题，拟利用高光谱成像技术和红外吸收光谱技术，进行非生物复合胁迫条件下小麦交叉响应机理的研究。开展非生物复合胁迫条件下, 小麦性状规律分析以及内源性甲烷产生与释放机制探索等研究工作。最终实现揭示非生物复合胁迫条件下，小麦交叉响应机理和非生物复合胁迫因素有效预测的目的。. 对非生物复合胁迫条件下小麦交叉响应机理进行分析，建立基于甲烷检测的非生物复合胁迫预测模型，为保证植物健康、高效生长提供理论依据和技术支持，不仅具有较高的科学研究价值，而且具有重要的现实意义。

针对非生物复合胁迫下小麦交叉响应机理尚未阐明，内源性甲烷(CH4)的释放规律没有明确，研究手段步骤繁琐、实时性差等问题。本课题以宁麦13及烟农0428小麦为研究对象，通过调节人工气候培养箱内温度实现高温胁迫、改变紫外线辐照灯照射强度实现紫外线胁迫、调节水培营养液NaCl浓度实现盐害胁迫、控制叶片上打孔的数量实现物理损伤胁迫。利用高光谱仪、叶面积仪、叶绿素荧光仪、光纤光谱仪等对小麦株高、叶面积、叶片光合参数、果胶含量等的响应特性进行分析。采用自主研发的基于红外吸收光谱技术的高灵敏度CH4传感器，对小麦样本内源性CH4释放规律进行探索。研究结果表明：非生物复合胁迫下，小麦的性状和内部相关物质的响应机制得以确定；内源性CH4的释放规律也已明晰。本研究能够为大田小麦非生物胁迫的探测和预防提供理论依据和技术支持，保证小麦健康、高效生长。