转HTFα对脊髓继发性损伤和微循环重建的影响

Biomolecular and immunohistologic techniques were adopted in this program to detect the expression of hypoxia-inducible factor 1 alpha (HIF-α) in protein and mRNA levels, in order to elucidate its effect and the corresponding mechanism to the spinal secondary injury and the spinal microcirculation reconstruction. It is suggested that HIF-α can be detected with high positive expression after the spinal injury which is the response to the ischemic hypoxia and correlated with the apoptosis medicated by Fas and Caspase-3 cell signal transduction pathway. TNF neutralizing antibody can suppress the apoptosis after the spinal injury, improve the injuried-spinal function and suppress the spinal neuron apoptosis in vitro. The expressions of glycolytic enzymes(LDH-M, ALDA ) ,iNOS and VEGF were increased remarkably which were all modulated by HIF-α. HIF-α can improve the glycolysis, vasodilation and microcirculation reconstruction, in order to let spinal neurons survive from the ischemic hypoxia environment. It is the specific self-protection mechanism of spinal neurons with the high expression level of HIF-α and its gene to manage the ischemic hypoxia circumstance. The combination of gene transfer with HIF-α which can increase the self-protection mechanism is a prosperous pathway to treat the spinal injury.

采用分子生物学及免疫组化方法观察HIF-1α在脊髓损伤后的表达；将含HIF-1α基因的重组原相关病毒转染脊髓神经细胞使目的基因持续高表达，研究HIF-1α对SD大鼠脊髓压迫伤后顾杓谭⑿运鸷退鹕撕蠹顾栉⒀分亟ǖ淖饔眉捌浞肿踊疲兰燮涠运鹕思顾璧男薷春蜕窬δ芑指吹募壑怠Ｎ剿骷跚峒顾杓谭⑿运鸷痛俳顾韫δ芑指刺峁┮恢中碌乃悸贰