硬脑膜动静脉瘘对脑微循环和脑功能影响的活体光学成像研究

The mechanism of brain micro-circulatory change and brain functional change after dural arteriovenous fistula is not clear. Through using the in vivo brain imaging techniques with high-spatiotemporal resolutions jointly, including laser speckle contrast imaging, endogenous signal optical imaging, and cortical somatosensory evoked potential technique, we attempted to monitor dynamic changes of micro-circulatory system and the cortical spreading depression after dural arteriovenous fistula, as well as the relationship between them, and the dynamic changes of hemodynamic indices and somatosensory evoked potential in cortex under the activated state induced by somatosensory electrical stimulation, as well as their impacts on the neurovascular coupling mechanism, with the objective to provide a new perspective for further understanding of pathological mechanism of brain micro-circulatory reestablishment and brain functional change after dural arteriovenous fistula, thus providing a theoretical basis for selecting new targets for its treatment.

硬脑膜动静脉瘘不同病程中脑微循环和脑功能的改变尚不甚明确。本课题拟应用高时空分辨率的活体光学脑功能成像技术:激光散斑成像、内源信号光学成像，动态监测硬脑膜动静脉瘘发生发展过程中硬脑膜及脑皮层微循环重构的时空变化过程，及其与皮层扩散性抑制之间的关系；联合光学成像与神经电生理技术，研究硬脑膜动静脉瘘对脑皮层功能及神经血管偶联的影响。本研究有望为更深入地理解硬脑膜动静脉瘘后脑血管重构及脑功能改变的病理机制提供新的视角，为针对其治疗选择新的靶点提供理论依据。

硬脑膜动静脉瘘参与了脑微循环和脑功能的重构。本课题通过高时空分辨率的活体光学脑功能成像技术:激光散斑成像、内源信号光学成像，动态监测硬脑膜动静脉瘘发生发展过程中硬脑膜及脑皮层微循环重构的时空变化过程。首先建立SD大鼠慢性窗模型与硬脑膜动静脉瘘模型，通过设置LCTF透过波长分别为550nm、630nm，利用CCD收集大鼠内源信号图像；进而采用660nm的LD灯照射在皮层上，利用CCD采集收集大鼠原始散斑图像。分别采集了硬脑膜动静脉瘘大鼠模型在1周、2周、4周和12周的图像。对收集的数据，利用RTLBI成像软件进行分析，发现在第1周、2周、4周，硬脑膜动静脉瘘确实改变了脑微循环和脑功能的重构，但12周的图像难以获得,最终无法观测到硬脑膜动静脉瘘的形成；监测硬脑膜动静脉瘘的皮层扩散性抑制动态过程以及体感刺激下大脑各项参数变化的工作还在进行。