抗bcl-2核酶(Ribozyme)诱导食管癌细胞凋亡的研究
基本信息
结项摘要
本研究利用核酶可序列特异地切割RNA的特点，设计并合成针对bcl-2 mRNA癌基因的锤头状核酶基因，体外测活表时，所设计核酶具有很强的定点切割bcl-2 mRNA的活性。我们先后构建了两 种真核表达载体PKOR-RZ 和PMTRN，前者为核酶基因的逆转录病毒表达载体，用以转染人早幼粒白血病细胞HL-60；后者为含金属硫蛋白基因启动子的可诱导型表达载体，用以转染人食管癌细胞Eca109.转染了PDOR-RZ的HL-60细胞伴随着核酶的表达而细胞Bcl-2蛋白水平下降，生长受抑制，流式细胞仪分析显示出凋亡峰，细胞DNA凝胶电泳呈现反映细胞凋亡特征的梯状条带，电镜下可见明显的细胞凋亡。PMTRN转染E ca109细胞后经ZnSO4诱导，亦可检测到有核酶的表达。
项目摘要
项目成果
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数据更新时间：2023-05-31
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