转录因子RAV1调控拟南芥低温应答的分子机制

Low temperature affects plant growth and development and leading to plant death. Arabidopsis transcription factor RAV1 is important in regulation of plant growth and development, as well as stress response. Previous study found that the transcriptional expression of RAV1 was induced by low temperature. However, the exact role of Arabidopsis RAV1 in cold stress response remains unclear. Our study found that the expression level of RAV1 affected the freezing tolerance of Arabidopsis. Based on this result, our study will focus on the RAV1 expression, target genes of RAV1 regulated, and freezing phenotype of transgenic plants, to identify the function of Arabidopsis RAV1 regulating cold signaling transduction. This study of RAV1 will shed new light on our understanding of the molecular mechanism of cold stress response in plant, and provide guidance for use of freezing-tolerant genes.

低温严重影响植物生长发育，甚至导致植物死亡。拟南芥RAV1是调控植物生长发育和逆境响应过程的重要转录因子。RAV1的转录水平受低温胁迫诱导表达，但是RAV1在植物低温胁迫响应过程中的作用机制尚不清楚。项目申请人前期研究发现RAV1的表达量影响拟南芥的抗冻性。项目申请人拟从RAV1的表达、RAV1调控的靶基因、以及相应的抗冻生理性状等方面开展研究，揭示拟南芥RAV1在低温信号转导中的作用，为进一步理解植物响应低温胁迫的分子机制和抗冻基因的发掘利用提供新思路和新途径，具有重要的理论研究意义和实际应用前景。