液相-气相二维色谱新技术研究多环芳烃和亚硝胺在吸烟者体内的代谢

It is well known that smoking is harmful to the health. However, to comprehensively understand the effects of smoking to health, the accurate determination the metabolisms of cigarette smoke harmful components in smoker body with high sensitive and selective modern analytical instruments is very important. The two dimensional chromatography technologies have it obvious advantages of high sensitivity and selectivity. In this project, the application of two-dimensional chromatography equipped with selected continuous cutting valve for the determination of polycyclic aromatic hydrocarbons and tobacco-specific nitrosamines in the body of cigarette smoker will be studied. The "liquid/gas two dimensional chromatography equipped with selected continuous cutting valve" will be designed based on the advantages of comprehensive two dimensional chromatography and liquid/gas two dimensional chromatography. The analysis methods of two kinds of harmful component (polycyclic aromatic hydrocarbons and tobacco-specific nitrosamines) in smoke will be established based on the new instrument.The liquid/gas two dimensional chromatography equipped with on-line solid phase extraction will also be designed, and the suitable solid-phase extraction materials for achieving the solid phase extraction of polycyclic aromatic hydrocarbons and tobacco-specific nitrosamines will be optimized.Based on these, the corresponding analytical method will be established. The polycyclic aromatic hydrocarbons and tobacco-specific nitrosamines in the blood and urine of smokers will be accurate determined by means of the new method. The metabolisms of polycyclic aromatic hydrocarbons and tobacco-specific nitrosamines in the body of smoker will be understanded by analysis data. This can give the evidence to understand the actual harm of the active and passive smoking on the health.

众所周知，吸烟有害健康。要深入了解吸烟对健康的影响，首先需了解烟气有害成分在吸烟者体内的代谢和分布情况。这必须利用高灵敏、高选择性的分析仪器和方法才有望实现。本项目拟在综合"全二维色谱"和"液相-气相二维色谱"优点的基础上，通过数控十通阀在线切换，设计出一种"可连续切割的液相-气相二维色谱装置"，该装置可克服传统液相-气相二维色谱进样量小，无法实现连续切割的缺点，充分发挥了两级色谱的互补性。由于两级色谱间带了在线浓缩(或富集)的功能，对于低含量样品可通过一级色谱多次进样累加，达到理想检测量后再进入第二级色谱分析。项目将依托新装置建立吸烟者尿液和血液中多环芳烃和亚硝胺的二维色谱分析方法，准确测定血液和尿液中两类有害成分含量，通过大量分析数据了解两类有害成分在吸烟者体内的代谢，了解不同吸烟者血液和尿液中的两类有害成分含量差异，从而更深入的了解吸烟对健康的实际危害。

本项目围卷烟主流烟气、吸烟者血液和尿液中以苯并[]芘为代表的多环芳烃和烟草特有亚硝胺分析进行了一系列的研究工作，并在综合“全二维色谱”和“液相-气相二维色谱”优点的基础上，通过数控十通阀在线切换，设计出一种“可连续切割的液相-气相二维色谱装置”，该装置可克服传统液相-气相二维色谱进样量小，无法实现连续切割的缺点，充分发挥了两级色谱的互补性。由于两级色谱间带了在线浓缩(或富集)的功能，对于低含量样品可通过一级色谱多次进样累加，达到理想检测量后再进入第二级色谱分析。项目将依托新装置建立吸烟者尿液和血液中多环芳烃和亚硝胺的二维色谱分析方法，准确测定血液和尿液中两类有害成分含量，通过大量分析数据了解两类有害成分在吸烟者体内的代谢，了解不同吸烟者血液和尿液中的两类有害成分含量差异。项目研究成果共发表(或接收)研究论文13篇，7篇论文为SCI或EI收录期刊，申请发明专利15件，获得授权发明专利2件，申请权实用新型专利5件，获授权2件。