华美曼蝽不同地理种群分化的研究（半翅目：异翅亚目：蝽科）

The geographic isolation and the distinct phenotype divergence were observed between Yunnan and Hainan populations of Menida speciosa Zheng & Xiong, 2001. We speculate that the phenotype divergence may have been reflected at the level of the gene and stable in heredity, which may lead to the cryptic species. Combining the comparative morphology, geometric morphometrics, pairwise cross-mating experiments and DNA barcoding techinique, this research aims to reveal the intraspecific differentiation degree and the biological mechanism of reproductive isolation by comparing the morphological difference, reproductive compatibility and genetic distance among the different populations and relative species. Also, we try to discuss the reliability of the genitalia as a key character to identify species in Pentatomidae (Hemiptera: Heteroptera). The results are meaningful to resolving the systematic relationships of intra- and inter-species,and clarifying the threshold between microevolution and macroevolution based on population and species, respectively, as the functional unit.

华美曼蝽Menida speciosa Zheng & Xiong, 2001的两个地理种群- - 云南种群和海南种群间存在地理隔离和显著的表型差异，我们推测这种表型分化可能已经反映到基因水平上，且能稳定遗传，可能有隐存种存在。本研究综合使用比较形态学、几何形态测量学、配对杂交实验和DNA条形码方法，通过比较华美曼蝽种群间及与近缘种间的形态差异、生殖兼容性和分子遗传距离，来揭示其种内分化水平和生殖隔离机制，并讨论雌雄生殖节作为判断异翅亚目蝽科物种间隔的可靠性。研究结果对解决种内和种间的系统分类问题、阐明异翅亚目以种群为功能单位的小进化和以物种为功能单位的大进化之间的阈值具有意义。

华美曼蝽（Menida speciosa Zheng & Xiong, 2001）的两个地理种群:云南种群和海南种群间存在地理隔离和显著的表型差异, 我们推测这种表型分化可能已经反映到基因水平上, 且能稳定遗传，可能有隐存种的存在。本研究综合运用比较形态分类、几何形态测量学、配对杂交实验和DNA 条形码方法，通过比较华美曼蝽种群间和与近缘种间的形态差异、生殖兼融性和分子遗传距离，揭示其种内分化水平和生殖隔离机制，并讨论雌雄生殖节作为判断异翅亚目蝽科物种间隔的可靠性。结果显示其具有表型差异、且不同种群不具备生殖隔离的条件、其种内分化水平在形态和分子上的表现是一致, 未发现隐存种。斑驳曼蝽M.mosaica与北曼蝽M.disjecta关系较近，分析中发现它们互为姊妹群，华美曼蝽M.speciosa与上述两种关系较近，分析中发现其与上述两种互为姊妹群。研究结果对解决种内和种间的系统分类问题、阐明异翅亚目以种群为功能单位的小进化和物种为功能单位的大进化之间的阈值具有意义。