刺葡萄花色苷分子间共-辅色条件及机制研究

Anthocyanins are a group of natural pigment which possess various physiological activity funtion, it's important to seek anthocyanin-rich plants and improve the stability of anthocyanins.The conditions and mechanism of intermolecular self-association and flavone copigmentation of anthocyanins are undefined,although its funtion is effective approach to stabilize anthocyanins. Taking anthocyanins from spine grape（Vitis davidii Fo?x.）as materials, imitating water-ethanol system, taking spectra parameters,CIE parameters and hydration kinetic parameters as index, the conditions of anthocyanins self-association will be investigated. The photo-thermal degradation law will be revealed. The relation between anthocyanin acyl and self-association effect will be explained. Through tracking and analyzing the molecular structure of self-associated anthocyanins and chromophore, the molecular mechanism of anthocyanins self-association will be explored, including the interaction mechanism between self-association and copigmentation. The results are expected to provide basis for color stabilization control technology of anthocyanins.

花色苷具有多种生理活性功能，也是一种安全的天然植物色素，寻求含量丰富的花色苷资源及提高其应用的稳定性意义重大。花色苷分子间自聚合共色和黄酮辅色作用是提高其稳定性的有效途径，但花色苷分子间共-辅色的作用条件和机理尚不明确。刺葡萄果皮含有丰富的花色苷，且以酰基化花色苷为主。本项目以刺葡萄花色苷为研究对象，模拟葡萄汁、葡萄酒的水、醇体系，以分光光谱、测色CIE系统及水合动力学参数为指标，探明花色苷自聚合的发生条件；揭示刺葡萄花色苷发生自聚合前后的光热降解规律；明确花色苷酰基化结构与自聚合效应之间的构效关系；通过现代分析手段跟踪花色苷自聚合分子结构和形态的变化，检测发色基团的分子信号，探索花色苷在水系和醇系中发生自聚合的分子机理；明确花色苷分子间共-辅色之间的相互作用机制。预期为花色苷色泽强化和稳定化调控技术提供依据。

提高花色苷的稳定性是扩大其应用的重要基础。花色苷分子间自聚合共色和黄酮辅色作用是提高其稳定性的有效途径，但花色苷分子间共-辅色的作用条件、稳定化效果和分子机理尚不明确，限制了花色苷分子间自聚合共色及辅色效应的应用。酰基化花色苷是一种稳定性较高的花色苷，其产生自聚合的条件及分子作用机制有待探明。刺葡萄果皮含有丰富的花色苷，且以酰基化花色苷为主。本项目以刺葡萄花色苷为研究对象，建立了刺葡萄花色苷高效分离及制备方法，获得酰基化花色苷单体；模拟葡萄汁、葡萄酒的水、醇体系，以分光光谱、测色CIE系统及水合动力学参数为指标，系统探明了酰基化花色苷自聚合的发生条件，包括浓度、温度、pH值、纯度等对花色苷自聚合度的影响；揭示了花色苷发生自聚合前后的光、热降解规律，建立了刺葡萄花色苷在光、热作用下花色苷含量变化的预测方程；分析了酚酸、黄酮类物质对花色苷辅色作用条件及效果，分别优化了水系条件下酚酸、黄酮对刺葡萄花色苷辅色的复合配方；通过现代分析手段跟踪花色苷自聚合分子结构和形态的变化，检测发色基团的分子信号，初步探索花色苷酰基化结构与自聚合之间的构效关系。研究结果可为花色苷色泽强化和稳定化调控技术提供一定的理论基础。