基于可降解的二维超薄纳米片载体构建协同抑菌药物及其活性研究

Bacterial infection has threatened human’s health. It is essentially important to develop novel therapeutic anti-bacterial agents which are able to effectively kills bacteria. This project designs and prepares biodegradable two-dimensional antibacterial ultra-thin nanosheets which co-load photosensitizer and silver nanoparticles as combined anti-bacterial nanoagents. Compared with traditional antibacterial drugs, this hybrid system has many merits. For example, the positive charge of two-dimensional antibacterial ultra-thin nanosheets facilitate the interaction with bacteria membrane, increase the delivery efficacy and reduce the dosage. The well-dispersion and isolation of photosensitizer and silver nanoparticles on the surface of nanocarriers can prevent self-aggregation. Moreover, the combination of two different anti-bacterial mechanisms (photodynamic treatment and silver nanoparticles) are promising to increase antibacterial efficacy and overcome drug resistance. This project aims to establish a feasible method to prepare two-dimensional ultra-thin nanosheets for combined antibacterial application, which is not only important in basic research, but also applicable to practical disinfection application.

长期以来，细菌感染是威胁居民健康和生命的重要疾病。开发能够有效对抗细菌感染的创新抑菌药物是急需解决的科学问题。本项目拟基于可降解的二维超薄纳米片为载体，设计开发一种包含银纳米颗粒和光敏剂两种不同抑菌机制的纳米抑菌药物，评价抑菌活性，并初步探索协同抑菌机理。相比于传统抑菌药物，此次构建的体系有众多优势。例如，二维结构超薄纳米片载体带有正电，可以有效地与细菌膜接触，提高抑菌有效成分与细菌作用效率；光敏剂分子和银纳米颗粒均匀分散在纳米载体表面，避免有效成分自身团聚失效；利用光动力疗法和银纳米颗粒两种不同的机理协同抑菌，可提高抑菌效率，有望攻克耐药菌问题。该项研究不仅在二维结构超薄抑菌纳米片制备技术与协同抑菌机理方面具有极其重要的基础科学价值，同时在抑菌药物研发领域有着重要的应用前景。

长期以来，细菌感染是威胁居民健康和生命的重要疾病。本项目成功开发了以光动力抑菌治疗为主的纳米抑菌药物，辅以银纳米颗粒的协同纳米抑菌体系。第一，本项目成功制备了二维层状双金属氢氧化物纳米薄片、二氧化硅纳米颗粒、金属-有机框架纳米结构等纳米抑菌制剂，并成功修饰上光敏剂二氢卟吩e6。第二，本项目采用各种表征技术，例如透射电镜、扫描电镜、傅立叶红外光谱仪等，详细地表征了制备的纳米抑菌制剂。第三，本项目完成了包括二维结构超薄纳米片在内的纳米抑菌材料性能评价，证明了所制备的纳米抑菌制剂有着极好的光稳定性好、单态氧产生效率高，抑菌性能优异，并对革兰氏阴/阳性菌和耐药菌有效。最后，本项目初步探索了抑菌机理，通过扫描共聚焦显微镜观察到了细菌内部活性氧簇的产生，证实了活性氧簇能够有效杀死细菌。本项目总共发表论文6篇，其中影响因子大于10论文一篇，影响因子大于5论文四篇，培养硕士研究生3名。