氮杂化合物及生物活性分子界面电荷转移研究新方法

The project which constructing the liquid/liquid interface simulation is based on bionic membrane and focus on studying bioactive porphyrin, mixed nitrogen compounds such as quinoxaline, antibiotic, cytochrome, biological enzyme and so on. In addition, Thin layer cyclic voltammetry (TLCV) combining with Scanning microscope electrochemical technology (SECM) is used to discuss the charge transfer dynamic process mechanism, Marcus reverse electronic regional theory and electricity catalytic mechanism when the bioactive molecule transfer on the L/L interface; by using computerized numerical simulation, density functional theory and chemometrics methods, the L/L interface charge transfer and catalytic mechanism can be perfect, establish more scientific and reasonable interface charge transfer dynamics model, provide a new method which apply to investigate the interface charge transfer process, analyse the dissolved nitrogen miscellaneous compounds accurately, simply and rapidly. This study have very important significance, it can promote much deeper research which is related to life system interface catalysis and the charge transfer research, but also a better understanding of the related physiological and pathological process.

本项目以具有生物活性的卟啉、喹喔啉等氮杂化合物及抗生素，细胞色素，生物酶等多种生物分子为研究对象，以构建液/液界面模拟仿生膜为研究基础，以简单、快速的薄层法循环伏安法（TLCV）辅以扫描电化学显微镜技术（SECM）相结合的研究手段，系统深入地探讨生物活性分子在液/液界面上的电荷转移动力学过程机理、电催化机理和Marcus反向电子区域理论；运用计算机数值模拟、密度泛函理论和化学计量学方法，完善液/液界面电荷转移及催化理论，建立更为科学合理的界面电荷转移动力学模型，提出一种适合于研究生命体系界面电荷转移过程和准确、简单、快速分析非水溶性氮杂化合物等生物活性物质的新方法。项目的实施对推动生命体系界面催化、电荷转移的深入研究，更好地认识相关的生理和病理过程都具有非常重要的意义。

本项目主要以构建液/液界面和固/液界面模拟仿生膜为基础，以简单、快速的薄层法循环伏安法（TLCV）电化学循环伏安法（CV）、差示脉冲伏安法（DPV）和扫描电化学显微镜技术（SECM）、紫外可见光谱（UV-vis）法相结合的研究手段，系统深入地研究了生物活性分子、辣椒素，硝基苯及其它硝基芳香污染物等在液/液界面和和固/液界面上的电荷转移动力学过程机理、电催化机理和Marcus反向电子区域理论；运用计算机数值模拟、密度泛函理论和化学计量学方法，完善液/液界面和固/液界面上电荷转移及催化理论，建立更为科学合理的界面电荷转移动力学模型，提出一种适合于研究生命体系界面电荷转移过程和提出了一种准确、简单、快速的分析氮杂化合物、生物活性物质和硝基芳香污染物的新方法、新技术。项目的实施对推动生命体系界面催化、电荷转移的深入研究，更好地认识相关的生理和病理过程都具有非常重要的意义。