赤拟谷盗肌球蛋白Dachs影响翅膀和腿发育的下游基因及其调控关系

Myosin Dachs works downstream of Fat signaling pathway to regulate wing and leg development in Drosophila melanogaster. However, current studies paid more attention on upstream genes of Dachs but less on its downstream genes, which makes downstream genes and their regulatory relationships of Dachs affecting wing and leg development unclear now. Thus, we dissect the effect of Dachs on wing and leg development in grain-stored pest Tribolium castaneum. RNA interference (RNAi), RNA-sequencing and co-immunoprecipitation will be performed to identify downstream genes of Dachs in Tribolium castaneum. Then we analyze their effect on wing and leg development and the regulatory relationships among them. This study not only plays fundamental roles in clarifying the mechanism of Dachs regulating insect wing and leg development but also provides the potential targets of insecticide for pest control.

肌球蛋白Dachs作用于Fat信号通路下游调控果蝇翅膀和腿的发育，但是目前的研究集中在Dachs的上游基因而极少关注它的下游基因，导致Dachs影响翅膀和腿发育的下游基因及其调控关系还不清楚。因此，本项目以储粮害虫赤拟谷盗为实验材料，解析Dachs对翅膀和腿发育的影响，利用RNA干扰、RNA-sequencing和免疫共沉淀技术鉴定Dachs的下游基因，分析它们对翅膀和腿发育的影响及它们之间的调控关系。本项目研究结果不仅为阐明Dachs调控昆虫翅膀和腿发育的机制打下基础，而且将为害虫防治提供潜在的杀虫剂靶标。

肌球蛋白Dachs作用于Fat信号通路下游调控果蝇翅膀和腿的发育，但是目前的研究集中在Dachs的上游基因而极少关注它的下游基因，导致Dachs影响翅膀和腿发育的下游信号通路还不清楚。本研究发现dachs对赤拟谷盗翅膀和腿的生长具有重要调控作用。RNA-seq结合RNA干扰等技术表明Lowfat作用于Dachs的下游调控翅膀和腿的生长，TOR通路的Thor和S6K也作为Dachs的下游基因发挥功能。本项目不仅初步解析了肌球蛋白Dachs调控昆虫翅膀和腿生长的机制，而且为针对昆虫生长和发育机制开发新的生物杀虫剂靶标提供理论依据。