高性能超氧负离子电致发光传感器的构建及用于细胞释放的实时检测

Electrochemiluminescence (ECL) sensors have been gained more and more attention with high sensitivity of chemiluminescence and easy operation of electrochemistry as an emerging detection technique, which show unique advantages in life science analysis field. Cell metabolism analysis as an important part of the life science analysis has been a research hot spot to biologists and analysts, however, because of its high activity, low concentration of metabolite, cell metabolism analysis has brought great challenges to the analysts. The aim of this project is to explore a series of novel methods and strategies for the analysis and detection of the important bioactive small molecules superoxide anion released from living cells by coupling a highly sensitive ECL sensor protocol with the development of nanotechnology and great advantage of nanamateials. Detailedly, this project consists of the following issues: (1) to set up high sensitive ECL sensor for detection of superoxide anion based on three-dimensional nanomaterials and real-time determination of superoxide anion released from living cells; (2) to develop cell in situ growth strategies based on luminol functional nanomaterials for real-time detection of superoxide anion released from living cells with ECL sensor; (3) to study the effect of common causing celluar damage drugs or protective drugs on stimulation or elimination superoxide anion and to explore the mechanism of drug action in molecular level in preliminary. More importantly, this project could open a new avenue in the real-time, in situ and quantative analysis of bioactive small molecules in cell metabolism and provide a convenient technical platform for studying of cellular injury mechanism and protective mechanism caused by drugs.

电致化学发光（ECL）传感器是一门新兴的检测技术，兼具了电化学的易操作性和化学发光的高灵敏性，在生命科学分析领域中表现出独特的优势。细胞代谢分析作为生命科学分析的重要内容之一，一直是研究的热点，但由于其代谢物活性大、浓度低，给分析工作者带来巨大挑战。本项目将ECL传感器引入到细胞代谢分析当中，以重要的活性物质超氧负离子为检测对象，结合纳米材料的巨大优势，开发一系列的用于细胞检测的超氧负离子ECL传感器。具体内容：（1）基于三维网状纳米材料构建一系列高灵敏超氧负离子ECL传感器及细胞超氧负离子释放的实时检测；（2）构建一系列可提供细胞原位生长的ECL传感器及超氧负离子释放的实时检测；（3）研究常见致细胞损伤及保护作用药物对超氧负离子含量的影响，初步从细胞水平探讨药物对细胞的作用机制。本研究成果有望为细胞代谢活性小分子检测提供新的检测平台，为研究药物对细胞损伤或保护作用分析提供新的技术平台。

细胞代谢分析作为生命科学分析的重要内容之一，一直是研究的热点，但由于其代谢物活性大、浓度低，给分析工作者带来巨大挑战。本项目将生物传感器引入到细胞代谢分析当中，以生命体中一些重要的活性物为检测对象，结合纳米材料的巨大优势，开发一系列的用于细胞检测的电化学及电化学发光传感器。主要完成了以下内容：（ⅰ）高性能电化学免疫传感器的构建及在肿瘤标志物检测中的应用。（ⅱ） “一锅法”制备聚鲁米诺复合纳米材料用于构建灵敏固态电化学发光传感器。（ⅲ）功能纳米材料增强生物传感器在生物活性分子检测中的应用。（ⅳ）高灵敏的电致发光传感器实现对细胞代谢小分子的实时、在线的检测。