OsAER61介导水稻稻瘟病抗性的分子机制研究

Highly resistant to rice blast is detected in Zhongzao 39, but its genetic basis is still unclear. In the previous study, a new gene, OsAER61, was obtained responding to rice blast resistance. Further studies confirmed that OsAER61 is localized on the cell membrane and the susceptible osaur61 line is accompanied by a decrease in salicylic acid content and an increase in jasmonic acid of that. Overexpression of transgenic lines showed significant enhancement of rice blast resistance. Based on previous studies, we are planning to further investigate into the function of OsAER61 C-terminal 495 amino acids; new metabolic pathways involved in the interaction with unknown proteins; the location of OsAER61 in Jasmonic acid and salicylic acid signal pathways, through protein interaction analysis; structure domain function analysis; transcriptome sequencing; RT-PCR; pull-down; BiFc and Co-IP , etc. In overall, this study aims to pinpoint the location of OsAER61 gene in a possible rice blast resistance pathway, and explain its role in the Jasmonic acid and salicylic acid signal pathways; It has a potential significance to interpretation of the genetic basis of rice blast resistance in Zhongzao 39.

超级稻中早39高抗稻瘟病，对其抗性遗传基础的研究尚不清楚。前期研究获得一个与稻瘟病抗性相关的新基因OsAER61。进一步研究证实，OsAER61定位于细胞膜上，感病osaer61伴随着水杨酸含量的下降和茉莉酸含量的升高。超表达转基因株系表现出对稻瘟病抗性的明显增强。本研究将在前期研究的基础上，继续以osaer61为材料，通过酵母双杂交实验、结构域分析、转录组测序、荧光定量、pull-down、BiFc和Co-IP等一系列细胞分子生物学实验技术，对OsAER61 C端495个氨基酸、未知互作蛋白等进行分析，探讨OsAER61 C端495个氨基酸行驶的功能，与未知蛋白互作参与的新代谢途径，以及OsAER61抗病性在水杨酸和茉莉酸信号途径中的位置，以期阐明该基因在水稻稻瘟病抗性分子调控机制中起的重要作用，初步诠释中早39抗稻瘟病遗传基础。

超级稻中早39高抗稻瘟病，对其抗性遗传基础的研究尚不清楚。前期研究获得一个与稻瘟病抗性相关的新基因OsAER61。研究表明，OsAER61定位于细胞膜上，感病osaer61伴随着水杨酸含量的下降和茉莉酸含量的升高，超表达转基因株系表现出对稻瘟病抗性的明显增强，OsAER61通过协调水杨酸和茉莉酸次生代谢通路行使对稻瘟病抗性的功能，外源喷施水杨酸可以增强稻瘟病抗性；在明确OsAER61赋予中早39稻瘟病抗性的基础上，利用中早39为骨干亲本，创制了聚合多抗性基因，且抗性增强的新材料；选育了4个具有重大推广潜力的新品种。