失水胁迫下月季切花RhMPK4影响花朵开放的机理解析

Cut rose is one of the most important cut flowers in the world. Water deficit stress is the main reason for abnormal rose petals, and leads to post-harvest losses. Mitogen-activated protein kinase cascades play an important role in the process of the signal transmission of various biotic and abiotic stress in plants. MPK4 play an important role in ROS, cold stress, salt stress. Osmotic stress and drought stress. However, there is no report about the effect of the gene on the opening of flowers under water deficit stress. We recently discovered that RhMPK4 quickly response to water deficit stress in gynoecia, which means RhMPK4 may involves in the signaling of water deficit stress and affects flower opening. This topic will further clarify the change of the amount of RhMPK4 protein and kinase activation, through western blot and In Gel kinase analysis experiment. And, VIGS was used to make clear the role of RhMPK4 in cut flower opening under water deficit stress. Results of the study will enhance the understanding of the role of RhMPK4 in flower opening under water deficit stress, promote the understanding of the effect of water deficit stress on flower opening. In practice, it will provide the basis for the development of new and efficient cut rose preservative.

月季切花是全球重要切花之一。失水胁迫是引起月季花瓣开放异常，导致采后损耗的主要原因。MAPK级联系统在植物的各种生物和非生物胁迫信号的传导过程中起着重要作用。MPK4主要在ROS，冷胁迫，盐胁迫，渗透胁迫和干旱胁迫中发挥作用。但关于该基因在失水胁迫下影响花朵开放的研究尚无报道。我们最近发现RhMPK4基因在雌蕊转录本上快速响应失水胁迫，提示RhMPK4很可能传递失水胁迫信号，参与了失水胁迫对花朵开放的影响。本课题拟通过western blot实验和在胶磷酸化实验，进一步明确RhMPK4蛋白和激酶活性在失水胁迫下花朵各组织中的变化，明确RhMPK4对失水胁迫的响应模型。结合VIGS方法，沉默RhMPK4，鉴定RhMPK4在失水胁迫过程中影响花朵开放的功能。研究结果将增强对RhMPK4在失水胁迫下影响花朵开放的功能认识，促进对失水胁迫影响月季花朵开放的作用机制的理解。

月季切花是全球重要切花之一。失水胁迫是引起月季花瓣开放异常，导致采后损耗的主要原因。MAPK级联系统在植物的各种生物和非生物胁迫信号的传导过程中起着重要作用。MPK4主要在ROS，冷胁迫，盐胁迫，渗透胁迫和干旱胁迫中发挥作用。我们克隆了RhMPK4基因的全长，检测了RhMPK4的失水胁迫的转录本，发现雌蕊RhMPK4转录本上快速响应失水胁迫，RhMPK4很可能传递失水胁迫信号，参与了失水胁迫对花朵开放的影响。构建了RhMPK4的原核表达体系，表达并纯化了RhMPK4蛋白。为研究RhMPK4在多个品种上是否具有相同机理，引种栽培了43个月季品种，并完成了其中12种的失水胁迫下表型观察。研究结果为揭示MAPK级联系统在失水胁迫过程中的传导机制奠定部分基础。