Kr-h1介导桔小实蝇生长发育的分子调控机制

The research focuses on the molecular action of Kr-h1 in growth and development of the oriental fruit fly, Bactrocera dorsalis, one of the most economically important fruit fly pests devastating fruit and vegetable industry. On the basis of previous study about identification of JH signal pathway genes and analysis of their expression pattern, a series of molecular techniques including RNAi, dual luciferase reporter assay, two-hybrid system and heterogeneous gene expression are utilized comprehensively to study the regulation function of transcription factors BdMet, BdTaiman and BdKr-h1: to identify the juvenile hormone response element of BdKr-h1 (kJHRE); to examine the interaction of BdMet, BdTaiman and kJHRE; to analyze transcription regulation of the genes in JH signal pathway; finally to clarify the molecular regulation mechanism of BdKr-h1 mediated in growth and development of the fruit fly. The results will enrich the knowledge about insect JH signal pathway, help understand the transcriptional regulation mechanism from B. dorsalis and other insects, promote to seek novel insecticide targets and provide new control strategy on the fruit flies. Therefore, it has important theoretical significance and application prospects.

本项目以果蔬危险性害虫桔小实蝇Bactrocera dorsalis为研究对象，紧密围绕BdKr-h1在桔小实蝇生长发育中的作用机理这一关键科学问题，综合运用RNAi、荧光素酶报告基因、哺乳动物双杂交系统、异源表达等技术和手段，在前期鉴定JH信号通路基因BdMet、BdTaiman和BdKr-h1并解析其时空表达模式的基础上，系统分析桔小实蝇JH信号通路基因间的转录调控；鉴定BdKr-h1启动区kJHRE元件；阐明转录因子BdMet、BdTaiman和kJHRE的功能互作；进而明确JH信号传导通路中的级联反应过程，最终揭示BdKr-h1介导桔小实蝇生长发育的分子调控机理。研究结果不仅有助于对昆虫JH信号传导通路的认识，促进理解桔小实蝇及其他昆虫生长发育的转录调控机制，还有利于寻找新的杀虫靶标，为实蝇类害虫防控提供新思路，因此具有重要的理论意义和应用前景。

本项目以果蔬危险性害虫桔小实蝇Bactrocera dorsalis为研究对象，紧密围绕BdKr-h1在桔小实蝇生长发育中的作用机理这一关键科学问题，在鉴定JH信号通路基因BdMet、BdTaiman和BdKr-h1并解析其时空表达模式的基础上，系统分析了桔小实蝇JH信号通路基因间的转录调控；明确了JH信号传导通路中的级联反应过程，最终揭示了BdKr-h1介导桔小实蝇生长发育的分子调控机理。研究结果不仅有助于对昆虫JH信号传导通路的认识，促进理解桔小实蝇及其他昆虫生长发育的转录调控机制，还发现BdKr-h1可作为潜在的杀虫靶标，为实蝇类害虫防控提供新思路，因此具有重要的理论意义和应用前景。项目实施期间，发表标注项目资助的论文13篇，其中SCI论文12篇；申请发明专利4项，授权2项；培养毕业2名博士生、4名硕士生；受邀参加3次国际学术会议；项目负责人获重庆市首批英才计划青年拔尖人才（2019）；获准2019年度重庆市科技进步奖一等奖1项（4/15）；此外，通过项目实施，培养毕业的2位研究生获重庆市优秀硕士学位论文，3位研究生获国家奖学金。