能量转移型肼的上转换纳米复合比率荧光探针的制备及荧光成像

As an important chemical reagent, hydrazine is widely used in pharmaceuticals, pesticides, corrosion inhibitors and textile dyes in various chemical industries. On the other hand, hydrazine is highly toxic and could be readily absorbed. With the increasing concern for health and the environmental pollution, more and more people pay attention to the study of hydrazine detection..In this project, through the clear design and synthesis of molecules, we try to clarify the inner relationship between structure and properties, and obtain several 1,8-naphthalimide-based molecular fluorescent probes towards hydrazine. Based on the above results, we want to prepare rare-earth upcoversion nanophosphores, whose emission spectra overlap the absorption spectra of the obtained small molecular fluorescent probes. We try to modify upcoversion nanophosphores by molecular fluorescent probes, summarize the influence factors of the organic small molecular structure, surface modification methods and size of nanoparticles on the energy transfer efficiency of the composite materials, and clarify the interaction mechanism of composite nanometer fluorescent probes with hydrazine in the intra cell or biological tissues. We will finally obtain near-infrared light-excited and luminescent resonance energy transfer-based nanocomposites as excellent selective and sensitive ratiometric fluorescent probes for hydrazine.

作为一种重要的化学试剂，肼被广泛应用于药物、农药、防腐剂和纺织染料等化工业，同时肼又是一种高毒性物质并且很容易被人体吸收。随着人类对健康和环境污染的日益关注，有关肼的检测研究越来越受到人们的广泛关注。.本课题拟以1,8-萘酰亚胺为母体，通过明确的分子设计与合成，揭示结构与性质的内在联系，得到对肼具有高灵敏性和高选择性的有机分子荧光探针。以此为基础，制备稀土上转换纳米粒子，使其发射光谱能够与分子探针的吸收光谱重叠。将有机分子荧光探针与稀土上转换纳米粒子进行键合，得到有机-无机复合纳米荧光探针，研究有机分子结构、纳米颗粒表面修饰方式和纳米粒子大小等对能量转移效率的影响，阐明复合纳米荧光探针与肼在细胞或生物组织内部相互作用的化学机制，最终得到近红外光激发、荧光共振能量转移型肼的高灵敏性、高选择性比率复合纳米荧光探针。

本项目通过优化实验条件，合成了以1,8-萘酰亚胺为母体和基于半菁的对肼有选择性识别的反应型有机分子荧光探针，研究了溶液中pH、金属离子、阴离子、生物小分子等对分子探针识别肼的影响，并研究了识别机制；在此工作基础上，我们又考察了分子荧光探针在细胞中的毒性、定位效果和对肼的识别效果；基于有机分子探针识别肼前后紫外-可见吸收光谱的变化，制备了具有良好形貌和较高发光量子产率的稀土上转换纳米颗粒；在以上工作基础上，将有机分子荧光探针与稀土上转换纳米粒子进行组装，得到了具有能量转移的有机-无机复合纳米材料，系统研究了溶液中复合材料的光稳定性、热稳定性和微环境对其识别效果的影响，并研究了细胞中复合材料的毒性、定位效果和发光识别效果。得到了近红外光激发、荧光共振能量转移型肼的高灵敏性、高选择性比率复合纳米荧光探针。