典型光活化杀虫剂对生物膜系统影响的毒理学研究

Based on the ultramicrostructure of midgut cell, permeability of cell membrane, Na+-K+-ATPase, and ion channel, a potencial light-activated insecticide, a-terthienyl (a-T), was studied on physiological and biochemical molecular mechanism of action against two important kinds of agricultural insect pests, Helicoverpa armigera and Ostrinia furnacalis, using whole-cell patch clamp technique, combined with modern physiological and biochemical zymological methods. The results indicated that midgut cells in 5th larvae of both of H. armigera and O. furnacalis were damaged, showing in pathological changes or injuries of the membrane system or subcellular structures, such as microvilli, mitochondria, endoplasmic reticulum, cell nuclear and basal membrane infolding, when feeding on sublethal dose of a-T for 24h and 48h under ultraviolet irradiation. Permeability of cell membrane in H. armigera can be raised by 49 percent. Na+-K+-ATPase from the heads of the two insects was inhibited by a-T both in vitro and in vivo whether light exposure or not, and the inhibited rate was corresponding to the treated concentrations and dosages. Ion channel H. armigera can also be affected by a-T with light exposure. The relationship among permeability of cell membrane, Na+-K+-ATPase, and ion channel demonstrated that cell membrane system is one of important target of light-activated insecticides against insect pests. Glutathione s-transferase (GSTs), glutathione peroxidase (GSH-PX), carboxylesterase (CarE), alkaline phosphatase (ALP), acid phosphatase (AP), and esterase isoenzyme could also be affected by a-T with different level.

首次采用现代电生理实验技术，结合生理生化酶学方法，研究光活化杀虫剂对重要农业害虫和卫生害虫生物膜系统的影响，分析比较药剂与膜通透性、ATP酶、突触后电位以及离子通乐涞哪谠诹担罢倚碌陌斜晡坏慊虬斜攴肿樱矫髌涠嚼硌ё饔没疲滦蜕锖侠砼┮┑纳杓朴氪粗疲峁┛蒲У睦砺垡谰荩哂兄匾砺奂壑岛凸憷τ们熬啊