新型生物材料结合干细胞治疗放疗后阴道损伤的疗效及机制研究

The incidence of cervical cancer is second only to breast cancer threat to women's health. Compared to other gynecological malignancies, the patients with cervical cancer is relatively young, most of them were in the time of sexually active when they were diagnosis. more than 70% patients can be alleviated by the standard treatment, and transferred to long-term follow-up. To be taken more radical pelvic external beam radiotherapy with or without brachytherapy. Radiation therapy, particularly brachytherapy, can cause irreversible damage to the vaginal tissue. Vaginal dryness, dyspareunia contracture, seriously affecting the quality of life. There is no right method to improve radiotherapy damage. This study intends to combine type I collagen and human adipose-derived mesenchymal stem cells, then the compounds were implanted to the damaged parts. By the physical and chemical properties of collagen, increase the survival time of stem cells in the body, increasing the stem cells in contact with the damaged parts of the area, in order to achieve the best therapeutic effect. To observe the effect of treatment in terms of the thickness of the vaginal epithelium, the expression of specific proteins, etc., and to explore its therapeutic mechanism. The study provides new experimental basis for the research and treatment of tissue damage after radiotherapy. Provide research foundation to improve the quality of life in patients with cervical cancer after radiotherapy, with good prospects for clinical application.

宫颈癌是发病率仅次于乳腺癌的威胁女性健康的恶性肿瘤。相比于其他妇科恶性肿瘤，宫颈癌患者相对年轻，而且70％以上患者经规范治疗可长期无瘤生存。放疗是宫颈癌的重要治疗方法。盆腔放疗，尤其是腔内放疗，会造成盆底组织不可逆性损伤。导致阴道干涩、挛缩，以致性交困难，严重影响生活质量。目前针对改善放疗后损伤的研究极为缺乏，缺乏有效的治疗方法。本研究拟将I型胶原蛋白与人脂肪间充质干细胞结合，将复合物植入受损部位，利用胶原蛋白的理化性质，提高干细胞在体内存活时间，增大干细胞与受损部位接触面积，以期治疗效果达到最佳。治疗后拟从阴道组织形态，特异性蛋白的表达等方面观察治疗效果，并探讨其治疗机制。此次研究为放疗后组织损伤的治疗提供新的试验依据和研究方向。为宫颈癌放疗后患者生存质量的改善提供研究基础，具有良好的临床应用前景。

宫颈癌是发病率仅次于乳腺癌的威胁女性健康的恶性肿瘤。近年来随着手术方式及放疗方法的进步，70％以上患者经规范治疗可长期无瘤生存。但是盆腔放疗，尤其是腔内放疗，会造成盆底组织不可逆性损伤。是·导致阴道干涩、挛缩，以致性交困难，严重影响生活质量。在这项研究中，我们将蛋白支架和大鼠脂肪间充质干细胞的复合物植入放疗后损伤的阴道组织中。发现该复合物能够促进阴道上皮细胞再生，修复阴道组织，并改善阴道狭窄和挛缩，而且治疗效果优于单独进行干细胞移植。干细胞与生物材料复合物治疗放疗后阴道损伤是可行的；并且 RNA-seq筛查提示移植入体内的ADSCs可能通过分泌外泌体激活或促进了阴道损伤组织的修复。