刺参消化道再生期间基因表达及细胞培养的研究

The regeneration of tissues and organs related to the regeneration of specific gene and protein expression is closely related to promote the proliferation of cell division to speed up the repair of tissues and organs.Marine invertebrates are infrequently used to turn natural regeneration related genes of the cell culture studies with cell line construction , so use a strong regenerative capacity of apostichopus japonicus were cultured for research animals , the optimized medium and culture conditions. Protein gene expression by inducing apostichopus japonicus spit dirty detection renewable difference (such as survivin and mortalin ) , regeneration gene and tissue and organ regeneration process of tissue layers and cell growth, distribution and variation ;The cloning of regeneration-related protein gene, turn the natural regeneration of tissue cells screened positive cell culture method to study cell proliferation and apoptosis , cell cycle, DNA replication , cell division, proliferation and differentiation of immortalized cell culture built on the study of human cell differentiation , the exact mechanism of tissue repair and stimulate the regeneration repair potential to lay the theoretical foundation for the marine invertebrate economic animals to build the cell lines provide a new theoretical basis , with a wide range of applications and economic value .

组织器官的再生与特异的基因和蛋白表达密切相关，且促进组织细胞分裂增殖加快修复组织器官。由于海洋无脊椎动物中很少利用转染再生相关基因研究细胞培养与建细胞系的相关研究，所以利用再生能力很强的刺参为研究动物进行原代培养、优化培养基和培养条件。通过诱导刺参吐脏检测再生差异表达蛋白基因（如survivin 和mortalin），研究再生相关蛋白基因及组织器官再生过程中各组织层和细胞的生长、分布等变化特征；克隆再生相关蛋白基因、转染再生组织细胞筛选阳性细胞培养的方法研究细胞增殖凋亡、细胞周期DNA 复制，细胞分裂增殖与分化并培养建永生化细胞，对研究人类的细胞分化、组织修复的确切机制和激发再生修复潜能奠定理论基础，为海洋无脊椎经济动物建细胞系提供新的理论依据，具有广泛的应用价值和经济价值。