食源性病原体与有害化学添加剂超灵敏分子检测的微纳生物传感分析方法

Foodborne diseases have become increasingly serious public health problems on food security not only in our country but also in the world. Based on our long-term research on biomedical sensing and immunoassay, taking common and harmful foodborne virus like Human enterovirus 71 (EV 71) of hand-foot-and-mouth disease and toxically chemical additive like clenobuterol hydrochloride in food as targets, the investigation on several scientific aspects of micro/nano biochips, such as effects of biophysics, controllable modification techniques for biomolecule interfaces, molecular engineering of bio-recognition receptors, and signal transformation of lower approach for molecular recognition, will be systematically conducted around their sensitivity, selectivity, reproducibility and stability for detection of virus, bacteria and chemical additive molecules. The project aims to construct some new biomedical sensing methods for the analysis of impedances of micro-gap array electrodes, probes of electroactive liposome, tunneling currents of ultrathin films, and nucleic acid replacement of nanotubes, and develop some novel sensing technologies and devices with high sensitivity, specificity, reliability and rapidity for detection of pathogens and their related antigen, antibody and gene molecules, especially for clenobuterol hydrochloride and EV 71, so that the ultrasensitive on-site inspection and early diagnosis of malignant foodborne diseases can be realized, and therefore contributions can be made to enhance the capability of healthcare sanitary quarantine inspection and science & technology competitiveness of biomedical diagnosis fields in our country.

食源性疾病已成为我国乃至世界在食品安全方面日益严重的公共卫生问题。本项目拟在长期从事生物医学传感与免疫分析的研究基础上，以常见的、危害性大的食源性疾病如手足口病中的肠道病毒71型（EV 71）和有毒食品化学添加剂如瘦肉精等为对象，围绕识别病毒、细菌、化学添加剂分子的微纳生物医学传感器的灵敏度、选择性、重现性和稳定性等问题，系统开展微纳器件的生物物理效应、生物分子界面可控修饰技术、生物识别受体的分子工程、分子识别下游信号转换等科学问题的探索研究，构建微间隙阵列电极电阻分析、电活性脂质体探针、超薄膜隧道电流分析、纳米管核酸置换分析等新型生物医学传感新方法，发展瘦肉精、肠道病毒71型等病原体抗原、抗体与基因分子检测的高灵敏性、高特异性、快速可靠的微纳生物医学传感技术和器件，实现恶性食源性疾病的超灵敏现场检测和早期诊断，为提升我国医疗卫生检验检疫能力与生物医学检测领域的科技竞争力做出贡献。

食源性疾病已成为我国乃至世界在食品安全方面日益严重的公共卫生问题。本项目拟在长期从事生物医学传感与免疫分析的研究基础上，以常见的、危害性大的食源性疾病关联的目标物如手足口病中的肠道病毒和有毒食品化学添加剂如瘦肉精（克伦特罗、莱克多巴胺、沙丁胺醇）、亚硝酸盐、溴酸盐、重金属等为对象，围绕识别有毒化学添加剂和肠道微生物分子、离子的微纳生物医学传感器的灵敏度、选择性、重现性和稳定性等问题，系统开展微纳器件的生物物理效应、生物分子界面可控修饰技术、生物识别受体的分子工程、分子识别下游信号转换等科学问题的探索研究，构建微间隙阵列电极电阻分析、电活性脂质体探针、超薄膜隧道电流分析、纳米管核酸置换分析等新型生物医学传感新方法，发展瘦肉精、亚硝酸盐、肠道微生物等病原体抗原、抗体与基因分子检测的高灵敏性、高特异性、快速可靠的微纳生物医学传感技术和器件，实现恶性食源性疾病的超灵敏现场检测和早期诊断，为提升我国医疗卫生检验检疫能力与生物医学检测领域的科技竞争力做出贡献。通过本项目的探索，已发表研究论文38篇，其中SCI源刊29篇；申请专利18项，获国家发明专利授权11项、实用新型专利6项；参加国际国内学术会议11次，应邀做口头报告8人次；《淡水鱼深加工关键技术研究与示范》获得湖南省科技进步一等奖；《纳米材料修饰电极的制备及用于食品中亚硝酸根检测的应用》获得湖南省优秀硕士学位论文奖；培养硕士研究生18名。