基于脊髓损伤研究夹脊电针调控细胞自噬－凋亡的作用机制

Cell Autophagy plays an important role in Secondary Spinal Cord Injury through inhibiting cell apoptosis. Previous studies have showed that Jiaji Electro-acupuncture can inhibit cell apoptosis after SCI (Spinal Cord Injury). It is one of the important mechanisms of effective treatment of Spinal Cord Injury. This study, based on SCI (Spinal Cord Injury), is to explore the molecular mechanism of Jiaji Electro-acupuncture regulating Cell Autophagy and further inhibiting Cell Apoptosis. Adopting Allen’s model, TEM (transmission electron microscope), Western Blot and RT-PCR for observing the histomorphology, ultrastructure, gene and protein expression of Autophagy related factors of the SCI (Spinal Cord Injury) rats’ neurons, this study is designed to illustrate the Jiaji Electro-acupuncture’s regulatory mechanism of Cell Autophagy of SCI (Spinal Cord Injury) rats, explore the neurobiological bases of Jiaji Electro-acupuncture’s definite therapeutic effect of treating SCI (Spinal Cord Injury), enrich scientific connotation of Electro-acupuncture treating SCI (Spinal Cord Injury) and provide theoretical foundation for the clinic.

细胞自噬在继发性脊髓损伤中发挥了重要作用，能够抑制细胞凋亡的发生。前期研究已经证实，夹脊电针同样能够抑制脊髓损伤后内质网应激介导的细胞凋亡，这是其有效治疗脊髓损伤的重要作用机制之一。本课题基于脊髓损伤，探讨夹脊电针调控细胞自噬，进而抑制内质网应激细胞凋亡的分子机制。本课题采用Allen’s模型，以透射电镜、Western Blot、 RT-PCR等技术，从细胞水平、分子水平观察夹脊电针对脊髓损伤大鼠神经细胞组织形态、超微结构、自噬相关因子的基因及蛋白表达，以期阐明夹脊电针对脊髓损伤大鼠细胞自噬的调控机制，探讨夹脊电针治疗脊髓损伤确切疗效的神经生物学基础，丰富电针治疗脊髓损伤的科学内涵，为临床推广提供理论依据。

细胞自噬在继发性脊髓损伤中发挥了重要作用，能够抑制细胞凋亡的发生。前期研究已经证实，夹脊电针同样能够抑制脊髓损伤后内质网应激介导的细胞凋亡，这是其有效治疗脊髓损伤的重要作用机制之一。本课题基于脊髓损伤，探讨夹脊电针调控细胞自噬，进而抑制内质网应激细胞凋亡的分子机制。本课题采用Allen’s模型，以透射电镜、Western Blot、RT-PCR等技术，从细胞水平、分子水平观察夹脊电针对脊髓损伤大鼠神经细胞组织形态、超微结构、自噬相关因子的基因及蛋白表达，证实了大鼠SCI后存在细胞自噬的发生，且可以形成完整自噬流；夹脊电针能够提高SCI大鼠脊髓组织自噬相关因子基因及蛋白的表达，促进细胞自噬，夹脊电针治疗SCI可能与促进局部组织细胞自噬的发生以及增强自噬流的动态过程相关。夹脊电针能够抑制SCI后的细胞凋亡。夹脊电针可能通过增加损伤组织细胞自噬的表达来拮抗细胞凋亡；而细胞自噬可能是SCI后神经细胞免于凋亡的自我保护机制之一。